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POLR1B Antibody - Azide and BSA Free

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Western Blot: POLR1B Antibody [NBP2-93757] - Analysis of extracts of various cell lines, using POLR1B at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
Western Blot: POLR1B Antibody [NBP2-93757] - Analysis of extracts of various cell lines, using POLR1B at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

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Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-93757-0.02ml
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POLR1B Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). IANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR1B
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for POLR1B Antibody - Azide and BSA Free

  • DNA-directed RNA polymerase I 135 kDa polypeptide
  • DNA-directed RNA polymerase I 135kDa polypeptide
  • DNA-directed RNA polymerase I subunit RPA2
  • EC 2.7.7.6
  • FLJ10816
  • FLJ21921
  • MGC131780
  • polymerase (RNA) I polypeptide B, 128kDa
  • RNA polymerase I subunit 2
  • RPA116
  • RPA135
  • RPA2
  • Rpo1-2

Background

POLR1B, also known as DNA-directed RNA polymerase I subunit RPA2, has a 1,135 amino acid long isoform that is approximately 128kDa, and a short 1,079 amino acid isoform that is approximately 121kDa. POLR1B is a RNA polymerase that is localized to the nucleolus. POLR1B is the second largest fundamental element of pol I, which is responsible for the production of ribosomal RNA. Current research on POLR1B is being performed in relation to several diseases and disorders including pneumonia, malaria and tuberculosis. POLR1B has also been shown to have interactions with Histone cluster 1 H4/A, H4/B, H4/C, H4/D and H4/E in pathways such as RNA Polymerase I transcription and RNA Polymerase III transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Bioinformatics

Gene Symbol POLR1B