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PNMT Antibody [Unconjugated]

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Western blot shows lysates of human adrenal gland tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PNMT at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human adrenal gland tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7854) followed by HRP-conjugated ...read more
PNMT was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Sheep Anti-Human PNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7854) at 5 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7854-SP
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PNMT Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PNMT
Ser2-Leu282
Accession # P11086
Specificity
Detects human PNMT in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) INMT and rhNNMT is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PNMT
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF7854 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PNMT Antibody [Unconjugated]

  • EC 2.1.1
  • EC 2.1.1.28
  • Noradrenaline N-methyltransferase
  • PENT
  • PENTMGC34570
  • phenylethanolamine N-methylase
  • phenylethanolamine N-methyltransferase
  • PNMT
  • PNMTase

Background

PNMT (Phenylethanolamine N-MethylTransferase; also PENT and Noradrenaline N-methyltransferase) is a monomeric, 30-34 kDa cytoplasmic member of the NNMT/PNMT/TEMT family of enzymes. It has limited expression, being found in Leydig, skeletal muscle and B cells, adrenal chromaffin cells, select adrenergic neurons of the vagus nerve, periventricular grey and reticular formation, and specialized adrenalin-containing cells contained within the heart. PNMT converts noradrenalin into adrenalin by transferring a methyl group from AdoMet to the terminal amine group of noradrenalin. Human PNMT is 282 amino acids (aa) in length and contains an active enzyme site between aa 14-279. Full length human PNMT1 shares 81% aa sequence identity with mouse PNMT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PNMT Antibody (AF7854)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol PNMT
Uniprot