Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688] - Analysis of extracts (10ug) from 9 Human tissue by using anti-PNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (1D2) [NBP2-00688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Western Blot: PNMT Antibody (1D2) [NBP2-00688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: PNMT Antibody (1D2) [NBP2-00688] Analysis of extracts (35ug) from 9 different cell lines by using anti-PNMT monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PNMT mouse monoclonal antibody.
30.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-00688 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for PNMT Antibody (OTI1D2)
EC 2.1.1
EC 2.1.1.28
Noradrenaline N-methyltransferase
PENT
PENTMGC34570
phenylethanolamine N-methylase
phenylethanolamine N-methyltransferase
PNMT
PNMTase
Background
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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