Novus Biologicals products are now on bio-techne.com

PNMT Antibody (OTI1D2)

Images

 
Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688] - Analysis of extracts (10ug) from 9 Human tissue by using anti-PNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (1D2) [NBP2-00688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Western Blot: PNMT Antibody (1D2) [NBP2-00688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: PNMT Antibody (1D2) [NBP2-00688] Analysis of extracts (35ug) from 9 different cell lines by using anti-PNMT monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PNMT mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

PNMT Antibody (OTI1D2) Summary

Immunogen
Full length human recombinant protein of human PNMT(NP_002677) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PNMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-00688 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PNMT Antibody (OTI1D2)

  • EC 2.1.1
  • EC 2.1.1.28
  • Noradrenaline N-methyltransferase
  • PENT
  • PENTMGC34570
  • phenylethanolamine N-methylase
  • phenylethanolamine N-methyltransferase
  • PNMT
  • PNMTase

Background

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP1-31386
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-36776
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-88527
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-93935
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NB100-1780
Species: Hu, Mu
Applications: Flow, PEP-ELISA, WB
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
AF2818
Species: Hu
Applications: ICC, WB
AF1936
Species: Hu
Applications: IP, WB
NBP1-89342
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-45570
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AF6457
Species: Hu
Applications: WB
NBP2-45946
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
DY4517-05
Species: Mu
Applications: ELISA
NBP2-67150
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB

Publications for PNMT Antibody (NBP2-00688)(2)

Reviews for PNMT Antibody (NBP2-00688) (0)

There are no reviews for PNMT Antibody (NBP2-00688). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PNMT Antibody (NBP2-00688) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PNMT Products

Research Areas for PNMT Antibody (NBP2-00688)

Find related products by research area.

Blogs on PNMT

There are no specific blogs for PNMT, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PNMT Antibody (OTI1D2) and receive a gift card or discount.

Bioinformatics

Gene Symbol PNMT
Entrez
Uniprot