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PLZF Antibody (6318100) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human PLZF Monoclonal Antibody (Catalog # MAB2944) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF ...read more
PLZF was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Human PLZF Monoclonal Antibody (Catalog # MAB2944) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
HL‑60 human acute promyelocytic leukemia cell line was stained with Human PLZF Monoclonal Antibody (Catalog # MAB2944, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
6318100
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2944U-100UG
Alexa Fluor 405
IC2944V-100UG
Alexa Fluor 488
IC2944G-100UG
Alexa Fluor 594
IC2944T-100UG
Alexa Fluor 647
IC2944R-100UG
Alexa Fluor 700
IC2944N-100UG
Alexa Fluor 750
IC2944S-100UG
Allophycocyanin
IC2944A
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC2944A
Carrier Free
MAB2944-SP
Carrier Free
IC2944V-100UG
Carrier Free
IC2944U-100UG
Carrier Free
IC2944T-100UG
Carrier Free
IC2944S-100UG
Carrier Free
IC2944R-100UG
Carrier Free
IC2944N-100UG
Carrier Free
IC2944G-100UG
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PLZF Antibody (6318100) [Unconjugated] Summary

Specificity
Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ZBTB16
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2944 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLZF Antibody (6318100) [Unconjugated]

  • PLZF
  • PLZFzinc finger and BTB domain-containing protein 16
  • ZBTB16
  • zinc finger and BTB domain containing 16
  • zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
  • Zinc finger protein 145
  • Zinc finger protein PLZF
  • ZNF145
  • ZNF145Promyelocytic leukemia zinc finger protein

Background

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PLZF Antibody
(6318100) [Unconjugat
MAB2944
PLZF Antibody (6318100) [Unconjugated]
Species: Hu
Applications: WB, CyTOF-ready, Flow, ICC/IF

Publications for PLZF Antibody (MAB2944)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC, IHC, IHC-P, Western Blot.


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ChIP
(1)
ICC
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(1)
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(2)
Mouse
(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZBTB16
Uniprot