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PLZF Antibodies

We offer PLZF Antibodies for use in common research applications: ELISA, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each PLZF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PLZF Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PLZF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PLZF polyclonal antibodies and browse our PLZF monoclonal antibody catalog.

Alternate Names for PLZF Antibodies

anti-PLZF antibody, anti-ZBTB16 antibody, anti-PLZFzinc finger and BTB domain-containing protein 16 antibody, anti-zinc finger and BTB domain containing 16 antibody, anti-zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia) antibody, anti-Zinc finger protein 145 antibody, anti-Zinc finger protein PLZF antibody, anti-ZNF145Promyelocytic leukemia zinc finger protein antibody

8 Results for "plzf" in Primary Antibodies

AF2944

PLZF Antibody [Unconjugated]

(42 Publications)

100 μg

AF2944

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Increased mTORC1 activity in germ cells from Lkb1 newborn cKO testis. (a) Western blot of testis proteins at different postnatal ages using specific antibodies against p-S6K1 (T389), p-rpS6 (S235/6), rpS6 and beta -tubulin. rpS6, and beta -tubulin were used as internal controls. (b) Expression of PI3K-, mTOR- and SPC-related markers by western blot in P8 testes from control and Lkb1 cKO mice. (c) Immunohistochemistry for VASA, GATA4, p-rpS6 in P8 control testis. (d) Immunohistochemistry for Lin28a and p-rpS6 in P8 control testis. Serial sections were used in order to compare cellular localizations of these markers in the same tubule. Asterisks, different distribution of Lin28a and p-rpS6 in the same tubule resulting in strong positive for Lin28a and weak staining for p-rpS6. Arrows, cells expressing both Lin28a and p-rpS6. (e) Immunohistochemistry for Plzf and p-rpS6 in control and cKO mice. (f) Mean number of Plzf- or p-rpS6-positive cells in each tubule (positive cells/total tubules). The data were shown as mean±S.E.M. of at least three replicates. *P<0.05. Scale bars=50 μm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.

Depletion of SPCs in Lkb1 cKO mice. Seminiferous tubules from 5- (a) and 13- (b) week-old control and Lkb1 cKO mice (n=3) were used for immunostaining using specific antibodies against PCNA, gamma H2AX and Plzf. Scale bars=50 μm. Arrows, representative Plzf-positive cells in tubules. (c) Quantification of Plzf-positive germ cells in each tubule. Data are presented as mean±S.E.M. **P < 0. 01 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2944

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2944-SP

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NBP1-80894

PLZF Antibody

(6 Publications)

0.1 ml

NBP1-80894

Western Blot: PLZF Antibody [NBP1-80894] - Analysis in human cell line HELA.

Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Pancreas shows weak nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Cerebral cortex shows moderate nuclear positivity in neuronal cells.

Immunohistochemistry-Paraffin: PLZF Antibody [NBP1-80894] - Staining of human Lung shows moderate nuclear positivity in macrophages.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB2944

PLZF Antibody (6318100) [Unconjugated]

(4 Publications)

100 μg

MAB2944

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human PLZF Monoclonal Antibody (Catalog # MAB2944) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PLZF was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Human PLZF Monoclonal Antibody (Catalog # MAB2944) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

HL‑60 human acute promyelocytic leukemia cell line was stained with Human PLZF Monoclonal Antibody (Catalog # MAB2944, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2944U-100UG
Alexa Fluor 405	IC2944V-100UG
Alexa Fluor 488	IC2944G-100UG
Alexa Fluor 532	FAB2944X
Alexa Fluor 594	IC2944T-100UG
Alexa Fluor 647	IC2944R-100UG
Alexa Fluor 700	IC2944N-100UG
Alexa Fluor 750	IC2944S-100UG
Allophycocyanin	IC2944A
Biotin	FAB2944B
CoraFluor 1	FAB2944CL1
DyLight 350	FAB2944D
DyLight 405	FAB2944E
DyLight 488	FAB2944K
DyLight 550	FAB2944L
DyLight 594	FAB2944M
DyLight 650	FAB2944W
DyLight 680	FAB2944Y
DyLight 755	FAB2944Z
FITC	FAB2944F
HRP	FAB2944H
Janelia Fluor 525	FAB2944JF525
Janelia Fluor 549	FAB2944I
Janelia Fluor 585	FAB2944JF585
Janelia Fluor 635	FAB2944JF635
Janelia Fluor 646	FAB2944J
Janelia Fluor 669	FAB2944JF669
PE	FAB2944P
mFluor Violet 450 SE	FAB2944MFV450
mFluor Violet 500 SE	FAB2944MFV500
mFluor Violet 610 SE	FAB2944MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC2944A
Carrier Free	MAB2944-SP
Carrier Free	IC2944V-100UG
Carrier Free	IC2944U-100UG
Carrier Free	IC2944T-100UG
Carrier Free	IC2944S-100UG
Carrier Free	IC2944R-100UG
Carrier Free	IC2944N-100UG
Carrier Free	IC2944G-100UG

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MAB8395

PLZF Antibody (816416)

100 μg

MAB8395

Western blot shows lysates of BaF3 mouse pro-B cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB8395) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLZF at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8395-SP

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MAB29441

PLZF Antibody (816421) [Unconjugated]

100 μg

MAB29441

Mouse splenocytes stimulated with Recombinant Mouse IL-12 (Catalog # 419-ML) and Recombinant Mouse IL-18 (Catalog # 9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB29441) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC83951U-100UG
Alexa Fluor 405	IC83951V-100UG
Alexa Fluor 488	IC83951G-100UG
Alexa Fluor 532	FAB29441X
Alexa Fluor 594	IC83951T-100UG
Alexa Fluor 647	IC83951R-100UG
Alexa Fluor 700	IC83951N-100UG
Alexa Fluor 750	IC83951S-100UG
Allophycocyanin	FAB29441A
Biotin	FAB29441B
CoraFluor 1	FAB29441CL1
DyLight 350	FAB29441D
DyLight 405	FAB29441E
DyLight 488	FAB29441K
DyLight 550	FAB29441L
DyLight 594	FAB29441M
DyLight 650	FAB29441W
DyLight 680	FAB29441Y
DyLight 755	FAB29441Z
FITC	FAB29441F
HRP	FAB29441H
Janelia Fluor 525	FAB29441JF525
Janelia Fluor 549	FAB29441I
Janelia Fluor 585	FAB29441JF585
Janelia Fluor 635	FAB29441JF635
Janelia Fluor 646	FAB29441J
Janelia Fluor 669	FAB29441JF669
PE	FAB29441P
PerCP	FAB29441C
mFluor Violet 450 SE	FAB29441MFV450
mFluor Violet 500 SE	FAB29441MFV500
mFluor Violet 610 SE	FAB29441MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC83951R-100UG
Carrier Free	MAB29441-SP
Carrier Free	IC83951S-100UG
Carrier Free	IC83951T-100UG
Carrier Free	IC83951V-100UG
Carrier Free	IC83951N-100UG
Carrier Free	IC83951G-100UG
Carrier Free	IC83951U-100UG

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H00007704-M01

PLZF Antibody (3A7)

0.1 mg

H00007704-M01

Western Blot: PLZF Antibody (3A7) [H00007704-M01] - Analysis of ZBTB16 expression in transfected 293T cell line by ZBTB16 monoclonal antibody (M01), clone 3A7.Lane 1: ZBTB16 transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: PLZF Antibody (3A7) [H00007704-M01] - Analysis of protein-protein interactions between TRAF2 and ZBTB16. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ZBTB16 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PLZF Antibody (3A7) [H00007704-M01] - Detection limit for recombinant GST tagged ZBTB16 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-19870

PLZF Antibody

0.1 ml

NBP2-19870

Western Blot: PLZF Antibody [NBP2-19870] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLZF antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PLZF Antibody [NBP2-19870] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLZF protein stained by PLZF antibody [N1N3] diluted at 1:2000. Blue: Hoechst 33342 staining.

Reactivity:

Hu, Ch, Mu, Rt, Bv, RM

Species Glossary

Reactivity: Hu, Ch, Mu, Rt, Bv, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-51632

PLZF Antibody (5B3) - BSA Free

0.025 ml

NBP1-51632

Western Blot: PLZF Antibody (5B3) [NBP1-51632] - Western blot analysis using ZBTB16 mouse mAb against Hela (1) cell lysate.

Immunocytochemistry/Immunofluorescence: PLZF Antibody (5B3) [NBP1-51632] - Immunofluorescence analysis of Hela cells using ZBTB16 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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