PLVAP Antibody - BSA Free

PLVAP (Plasmalemma vesicle associated protein) is a membrane associated protein of caveolae and is found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Normally in skeletal and cardiac muscle, PLVAP expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of PLVAP expression in the CNS may be associated with the establishment of the blood brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re expression of the panendothelial cell antigen. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: EM, IHC-P, Immunohistochemistry-Paraffin, WB.

Showing Publications 1 - 6 of 6.

Publications using NBP1-83911	Applications	Species
Shuhan W, Jinxiao L, Luorui S, Liuying C et Al. Dachengqi decoction ameliorated liver injury in liver fibrosis mice by maintaining gut vascular barrier integrity Phytomedicine 2024-11-22 [PMID: 39577253]
Wilkinson A, Hulme S, Kennedy J et al. The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration iScience 2023-09-01 [PMID: 37810232]
Anri S, Kunio K, Yuto I et al. Overexpression of Plasmalemmal Vesicle-Associated Protein-1 Reflects Glomerular Endothelial Injury in Cases of Proliferative Glomerulonephritis with Monoclonal IgG Deposits Kidney Int Rep. 2022-10-19 [PMID: 36644361] (Immunohistochemistry-Paraffin, Human)	Immunohistochemistry-Paraffin	Human
Yokomori H, Ando W, Oda M Plasmalemmal Vesicle-Associated Protein Is Associated with Endothelial Cells Sprouting from the Peribiliary Capillary Plexus in Human Cirrhotic Liver Journal of vascular research 2021-07-19 [PMID: 34280928] (IHC-P, EM, WB)	IHC-P, EM, WB
Xie Y, He L, Lugano R Et Al. Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI insight 2021-07-06 [PMID: 34228647] (IHC-P)	IHC-P
Baba T, Grebe R, Hasegawa T et al. Maturation of the fetal human choriocapillaris. Invest Ophthalmol Vis Sci 2009-07-01 [PMID: 19264887]

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Wendy Dailey	In-Cell Western	Human	03/14/2019	View Summary Application Other Sample Tested Human retinal endothelial cells and U937 Species Human Lot B115774
Enlarge	4 reviewed by: Wendy Dailey	WB	Human	02/26/2019	View Summary Application Western Blot Sample Tested Human retinal endothelial cells Species Human Lot B115774 Comments Comments Cell lysate from Human Retinal Microvascular Endothelial Cells (HRMECs) was prepared and loaded onto Mini-protean TGX gel followed by western blot with NBP1-83911 (PLVAP antibody) at a dilution of (1:1000) and incubated over night at 4 C. Left = untreated cells and right = 24 hr VEGFA treatment. Green band = PLVAP and Red band = Actin
Enlarge	5 reviewed by: Santhosh Sivajothi	IHC-P	Human	05/22/2018	View Summary Application Immunohistochemistry-Paraffin Sample Tested Human kidney Species Human Lot A83158 Comments Comments Heat mediated antigen retrieval was performed by heating in citrate buffer (pH6) at 95C for 20 minutes

Array NBP1-83911

• PLVAP Antibodies
• PLVAP ELISA Kits
• PLVAP Lysate
• PLVAP Proteins