Name: Occludin Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-87402
Rating: 4 (4 reviews)

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Images

Ratings

Applications

Species

Date

Details

reviewed by:
Russ Tas

ICC

Rat

04/06/2022

View

Summary

Application	Immunocytochemistry
Sample Tested	IEC-6 rat intestinal epithelial cells
Species	Rat
Lot	10666

reviewed by:
Verified Customer

ELISA

06/01/2021

Application	ELISA
Sample Tested	rat intestine
Species	Rat
Lot	000010666

Enlarge

reviewed by:
James Xiao

Mouse

06/15/2017

Application	Western Blot
Sample Tested	Primary lung endothelial cells
Species	Mouse
Lot	B106982

Comments

Membrane was blocked in 5% milk in TBST. Primary antibody dilution was 1:1000 and the dilution for the secondary ab was 1:5000.

04/06/2015

Application	Immunofluorescence
Sample Tested	Mouse choroid plexus cells
Species	Mouse

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, IHC, KD
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free

Catalog# & Formulation	Size	Price
BSA Free NBP1-87402-25ul

Immunogen	This antibody was developed against Recombinant Protein corresponding to amino acids: DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED
Marker	Tight Junctions Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	OCLN
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.2), 40% Glycerol
Preservative	0.02% Sodium Azide
Purity	Immunogen affinity purified

Occludin Antibody - BSA Free

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Publications for Occludin Antibody (NBP1-87402)(25)

Reviews for Occludin Antibody (NBP1-87402) (4) 44

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Bioinformatics

Publications using NBP1-87402	Applications	Species
Greene C, Rebergue N, Fewell G et Al. NX210c drug candidate peptide strengthens mouse and human blood-brain barriers Fluids Barriers CNS 2024-09-27 [PMID: 39334382]
Lesha Pretorius, Carine Smith Green rooibos (Aspalathus linearis) promotes gut health: insight into mechanisms. Journal of ethnopharmacology 2023-11-21 [PMID: 37923252]
Wang S, Zhou J, Lu J et al. A ketogenic diet improves vascular hyperpermeability in type 2 diabetic mice by downregulating vascular pescadillo1 expression Journal of cellular and molecular medicine 2023-04-15 [PMID: 37060584] (WB, Mouse) Details: 1:500 WB dilution	WB	Mouse

Hamade DF, Epperly MW, Fisher R et al. Release of Interferon-? (IFN-?) from Probiotic Limosilactobacillus reuteri-IFN-? (LR-IFN-?) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS) following Whole Abdominal Irradiation Cancers 2023-03-08 [PMID: 36980556] (IHC, Mouse)	IHC	Mouse
Pretorius L, Smith C. Aspalathus linearis (Rooibos) and Agmatine May Act Synergistically to Beneficially Modulate Intestinal Tight Junction Integrity and Inflammatory Profile Pharmaceuticals (Basel) 2022-09-01 [PMID: 36145318]
Kansakar U, Gambardella J, Varzideh F et al. miR-142 Targets TIM-1 in Human Endothelial Cells: Potential Implications for Stroke, COVID-19, Zika, Ebola, Dengue, and Other Viral Infections International Journal of Molecular Sciences 2022-09-06 [PMID: 36142146] (Western Blot)	Western Blot
Baumann V Identification and manipulation of chromatin barriers in transcriptional reprogramming Thesis (ICC/IF)	ICC/IF
Werawatganon, D;Vivatvakin, S;Somanawat, K;Tumwasorn, S;Klaikeaw, N;Siriviriyakul, P;Chayanupatkul, M; Effects of probiotics on pancreatic inflammation and intestinal integrity in mice with acute pancreatitis BMC complementary medicine and therapies 2023-05-22 [PMID: 37217916] (IHC-P, Mouse)	IHC-P	Mouse
Li Y, Wei JY, Liu H et al. An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity Cell reports 2022-04-12 [PMID: 35417709] (ICC/IF, Human)	ICC/IF	Human
Pretorius L, Van Staden ADP, Van der Merwe JJ et al. Alterations to microbial secretome by estrogen may contribute to sex bias in irritable bowel syndrome Inflammopharmacology 2022-01-13 [PMID: 35022916]
Hua Q, Han Y, Zhao H et al. Punicalagin alleviates renal injury via the gut-kidney axis in high-fat diet-induced diabetic mice Food & function 2022-01-06 [PMID: 34989745] (IHC-P)	IHC-P
Marika Haderer, Philip Neubert, Eva Rinner, Annika Scholtis, Lucile Broncy, Heidi Gschwendtner, Arne Kandulski, Vlad Pavel, Alexander Mehrl, Christoph Brochhausen, Sophie Schlosser, Karsten Gülow, Claudia Kunst, Martina Müller Novel pathomechanism for spontaneous bacterial peritonitis: disruption of cell junctions by cellular and bacterial proteases Gut 2022-03-01 [PMID: 33707230]
Delaney C, Farrell M, Doherty CP et al. Attenuated CSF-1R signalling drives cerebrovascular pathology EMBO molecular medicine 2020-12-22 [PMID: 33350588] (WB, Mouse, Human)	WB	Mouse, Human
Patterson L, Allen J, Posey I et al. Glucosylceramide production maintains colon integrity in response to Bacteroides fragilis toxin-induced colon epithelial cell signaling FASEB J 2020-10-13 [PMID: 33047400]
Heldt NA, Seliga A, Winfield M et al. Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation Brain Behav. Immun. 2020-03-31 [PMID: 32243899] (IF/IHC, Mouse)	IF/IHC	Mouse
Shi Y, Li R, Yang J et al. No tight junctions in tight junction protein-1 expressing HeLa and fibroblast cells Int J Physiol Pathophysiol Pharmacol 2020-05-19 [PMID: 32067099] (IF/IHC, Mouse)	IF/IHC	Mouse
Shastri S, Shinde T, Sohal SS et al. Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms Int J Mol Sci 2020-01-12 [PMID: 31940911] (IF/IHC, Mouse)	IF/IHC	Mouse
Almassy J, Diszhazi G, Skaliczki M et al. Expression of BK channels and Na+-K+ pumps in the apical membrane of lacrimal acinar cells suggests a new molecular mechanism for primary tear-secretion. Ocul Surf 2019-01-24 [PMID: 30685438] (ICC/IF, Mouse)	ICC/IF	Mouse
Messal N, Fernandez N, Dayot S et al. Ectopic expression of OX1R in ulcerative colitis mediates anti-inflammatory effect of orexin-A Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018-11-01 [PMID: 30251681] (WB, Mouse)	WB	Mouse
Rom S, Zuluaga-Ramirez V, Gajghate S et al. Hyperglycemia-Driven Neuroinflammation Compromises BBB Leading to Memory Loss in Both Diabetes Mellitus (DM) Type 1 and Type 2 Mouse Models. Mol. Neurobiol. 2018-07-05 [PMID: 29974394] (IF/IHC)	IF/IHC
Mikula-Pietrasik J, Uruski P, Szubert S et al. Malignant ascites determine the transmesothelial invasion of ovarian cancer cells Int. J. Biochem. Cell Biol. 2017-09-06 [PMID: 28888784] (IHC-P, Human)	IHC-P	Human
Brychka D, Ayala-Nunez N, Bare Y et al. Targeting monocytic Occludin impairs monocyte transmigration and HIV neuroinvasion bioRxiv 2023-09-12 (ICC/IF, Human)	ICC/IF	Human
Evran S, Calis F, Akkaya E et al. The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model Brain Res. Bull. 2019-11-09 [PMID: 31715313] (IF/IHC, Rat)	IF/IHC	Rat
Li H, Sun J, Du J et al. Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut-brain axis. Neurogastroenterol Motil 2018-05-01 [PMID: 29193450]
Show All 25 Publications. Collapse Publications.