![Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856930.jpg "Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | M535 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Clone M535 was generated from a Plexin A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin A1. This sequence is highly conserved in rat and mouse Plexin A1, and has low homology to other Plexin family members. [UniProt# Q9UIW2] |
| Specificity | The antibody detects a 220 kDa protein corresponding to the apparent molecular mass of Plexin A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PLXNA1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21496.jpg)
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21497.jpg)
![Western Blot Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Western_Blot_21557.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Plexin A1 Antibody (NBP3-23316)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PLXNA1 |
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856954.jpg "Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg)
![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![Western Blot Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Western_Blot_5394.jpg)
![Immunohistochemistry Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Immunohistochemistry_6819.jpg)
![Simple Western Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Simple_Western_20498.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3A_1250S3_1473.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")