Home » Cyr61/CCN1 » Cyr61/CCN1 Antibodies » Cyr61/CCN1 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Cyr61/CCN1 Antibody [Unconjugated]

 (13 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 ...read more
Cyr61/CCN1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 51 kDa (as indicated) using 10 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4055-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Cyr61/CCN1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Cyr61/CCN1
Asp176-Gly281
Accession # NP_034646
Specificity
Detects mouse Cyr61/CCN1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human Cyr61/CCN1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CYR61
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4055 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyr61/CCN1 Antibody [Unconjugated]

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cyr61
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1

Background

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated protein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176‑281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CTGF/CCN2 Antibody
[Unconjugated] - C-te ...
AF660
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus
(7 Publications)
Species: Hu
Applications: IHC
NOV/CCN3 Antibody
[Unconjugated]
AF1640
Immunohistochemistry NOV/CCN3 Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
Plexin A1 Antibody
[Unconjugated]
AF4309
Immunohistochemistry Plexin A1 Antibody [Unconjugated]
Immunocytochemistry Plexin A1 Antibody [Unconjugated]
Western Blot Plexin A1 Antibody [Unconjugated]
(12 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
RPL10 Antibody
NBP1-84037
Immunohistochemistry-Paraffin RPL10 Antibody
Immunohistochemistry-Paraffin RPL10 Antibody
Immunohistochemistry-Paraffin RPL10 Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(675 Publications)
Species: Hu
Applications: ELISA
WISP2 Antibody -
Azide and BSA Free
NBP2-93612
Immunohistochemistry-Paraffin WISP2 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin WISP2 Antibody - Azide and BSA Free
Western Blot WISP2 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
WISP-1/CCN4
Antibody
AF1680
Immunohistochemistry WISP-1/CCN4 Antibody
(3 Publications)
Species: Mu
Applications: IHC, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(19 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Integrin alpha V
beta 3 [Unconjugated]
3050-AV
Integrin alpha V beta 3 [Unconjugated]
(25 Publications)
Species: Hu
Applications: Bind
EGR1 Antibody
[Unconjugated]
AF2818
EGR1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ICC, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(531 Publications)
Species: Hu
Applications: BA
FosB/G0S3 Antibody
[Unconjugated]
AF2214
Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]
Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]
Species: Hu
Applications: ICC, IHC, WB
Cyr61/CCN1
Antibody [Unconjugate
AF4055
Cyr61/CCN1 Antibody [Unconjugated]
Species: Mu
Applications: WB, Simple Western, IHC

Publications for Cyr61/CCN1 Antibody (AF4055)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Direct ELISA, IHC-Fr, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Direct ELISA
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF4055 Applications Species
Menger, MM;Bauer, D;Bleimehl, M;Scheuer, C;Braun, BJ;Herath, SC;Rollmann, MF;Menger, MD;Laschke, MW;Histing, T; Sildenafil, a phosphodiesterase-5 inhibitor, stimulates angiogenesis and bone regeneration in an atrophic non-union model in mice Journal of translational medicine 2023-09-08 [PMID: 37684656] (Mouse) Mouse
N Cheng, KH Kim, LF Lau Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2 JCI Insight, 2022-06-16;0(0):. 2022-06-16 [PMID: 35708907] (Western Blot, Mouse) Western Blot Mouse
Shuang Ma, Ryan Kanai, Ajaybabu V. Pobbati, Shuo Li, Kepeng Che, Caleb N. Seavey et al. The TAZ-CAMTA1 Fusion Protein Promotes Tumorigenesis via Connective Tissue Growth Factor and Ras–MAPK Signaling in Epithelioid Hemangioendothelioma Clinical Cancer Research 2022-07-15 [PMID: 35443056]
Jing Ren, Xiaoyu Wang, Sarah N. Parry, Christine Yee, Mark D. Gorrell, Susan V. McLennan et al. Targeting CCN2 protects against progressive non-alcoholic steatohepatitis in a preclinical model induced by high-fat feeding and type 2 diabetes Journal of Cell Communication and Signaling 2022-09-01 [PMID: 35038159]
Nicole R. Wong, Jay Mohan, Benjamin J. Kopecky, Shuchi Guo, Lixia Du, Jamison Leid et al. Resident cardiac macrophages mediate adaptive myocardial remodeling Immunity 2021-09-01 [PMID: 34320366]
MM Menger, B Merscher, C Scheuer, BJ Braun, SC Herath, MF Rollmann, D Stenger, T Später, T Pohlemann, MD Menger, T Histing Amlodipine accelerates bone healing in a stable closed femoral fracture model in mice European cells & materials, 2021-05-24;41(0):592-602. 2021-05-24 [PMID: 34027631] (Western Blot, Mouse) Western Blot Mouse
D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury Sci Rep, 2019-11-13;9(1):16736. 2019-11-13 [PMID: 31723159] (Western Blot, Mouse) Western Blot Mouse
Feng Hao, Fuqiang Zhang, Daniel Dongwei Wu, Dong An, Jing Shi, Guohong Li et al. Lysophosphatidic acid-induced vascular neointimal formation in mouse carotid arteries is mediated by the matricellular protein CCN1/Cyr61 American Journal of Physiology-Cell Physiology 2016-12-01 [PMID: 27760754]
CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56. 2016-08-01 [PMID: 27482114] (Direct ELISA, IHC-Fr, Neutralization, Mouse) Direct ELISA, IHC-Fr, Neutralization Mouse
Kim K, Chen C, Alpini G, Lau L CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB. J Clin Invest, 2015-03-30;125(5):1886-900. 2015-03-30 [PMID: 25822023] (Western Blot, Mouse) Western Blot Mouse
Show All 13 Publications.

Reviews for Cyr61/CCN1 Antibody (AF4055) (0)

There are no reviews for Cyr61/CCN1 Antibody (AF4055). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cyr61/CCN1 Antibody (AF4055) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cyr61/CCN1 Products

Blogs on Cyr61/CCN1

There are no specific blogs for Cyr61/CCN1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.
Sheep IgG Isotype Control [Unconjugated]
5-001-A
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cyr61/CCN1 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CYR61
Uniprot