![Western Blot: Plasminogen Antibody [NBP2-19859] - Rat tissue extract (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0012.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Rat tissue extract (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Plasminogen. The exact sequence is proprietary. |
| Localization | Secreted |
| Predicted Species | Rhesus Macaque (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLG |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Knockout Validated SerpinB2 Antibody (930109) [Unconjugated]](https://images.novusbio.com/images2/Serpin_B2_MAB8550_Knockout_Validated_21056.jpg)
![Immunocytochemistry SerpinB2 Antibody (930109) [Unconjugated]](https://images.novusbio.com/images2/Serpin_B2_MAB8550_Immunocytochemistry__Immunofluorescence_17162.jpg)
![Western Blot SerpinB2 Antibody (930109) [Unconjugated]](https://images.novusbio.com/images2/Serpin_B2_MAB8550_Western_Blot_17242.jpg)
| Publication using NBP2-19859 | Applications | Species |
|---|---|---|
| Burchall GF, Pouniotis DS, Teede HJ et al. Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state Reprod. Biol. Endocrinol. 2019-03-16 [PMID: 30878040] (IHC-P, Mouse) | IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Plasminogen Antibody (NBP2-19859)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Plasminogen Antibody [NBP2-19859] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19859-img0006.jpg "Immunocytochemistry/Immunofluorescence: Plasminogen Antibody [NBP2-19859] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-NBP2-19859-img0013.jpg "Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0001.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0002.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0003.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0009.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0010.jpg "Western Blot: Plasminogen Antibody [NBP2-19859] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0004.jpg "Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0005.jpg "Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0008.jpg "Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.")
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![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A uPAR [HRP]](https://images.novusbio.com/images2/DATA_uPAR_DUP00_ELISA_866.jpg)
![N/A uPAR [HRP]](https://images.novusbio.com/images2/uPAR_DUP00_ELISA_203.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")