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PKA C alpha/beta Antibody (515741)

 (4 Publications)
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Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PKA Ca/ beta was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse ...read more
Western blot shows Recombinant Human Active PKA Ca (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (1 ng/lane each). PVDF membrane was probed with 0.2 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
515741
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5908-SP
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PKA C alpha/beta Antibody (515741) Summary

Immunogen
E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612
Specificity
Detects human, mouse, and rat PKA C alpha / beta in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRKACA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
MAB5908 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKA C alpha/beta Antibody (515741)

  • CAFD2
  • PKA C alpha/beta
  • PKA C-beta
  • PKACB
  • PRKACA/B

Background

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha , C beta , and C gamma , have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PKA C alpha/beta
Antibody (515741)
MAB5908
PKA C alpha/beta Antibody (515741)
Species: Hu, Mu, Rt
Applications: WB

Publications for PKA C alpha/beta Antibody (MAB5908)(4)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Western Blot.


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(2)
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Bioinformatics

Gene Symbol PRKACA
Uniprot