![Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PIWIL1-HIWI-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06062-img0002.jpg "Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-SNRKDKYDAIKKY corresponding to internal region according to NP_004755.2, NP_001177900.1. |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1). |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PIWIL1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PIWIL1 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062] - Staining of paraffin embedded Human Testis. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/PIWIL1-HIWI-Antibody-Immunohistochemistry-Paraffin-NBP1-06062-img0003.jpg "Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062] - Staining of paraffin embedded Human Testis. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PIWIL1-HIWI-Antibody-Flow-Cytometry-NBP1-06062-img0004.jpg "Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PIWIL1-HIWI-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06062-img0001.jpg "Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
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