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Periostin/OSF-2 Antibody (OTI1A1)

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Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.
Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73335
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Periostin/OSF-2 Antibody (OTI1A1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-230 of human Periostin/OSF-2 (NP_006466) produced in E.coli.
Specificity
This antibody will possibly detect all isoforms encoding the amino acid regions 1-230 of human Periostin/OSF-2.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
POSTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry Reactivity reported in (PMID:22512703).
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Theoretical MW
93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47922 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Periostin/OSF-2 Antibody (OTI1A1)

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta 1(TGF beta 1) and bone morphogenetic protein (BMP2). Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Abnormal expression of periostin is also linked to angiogenesis and metastatsis in epithelial tumors

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Periostin/OSF-2 Antibody (NBP1-47922)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


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Bioinformatics

Gene Symbol POSTN
Entrez
Uniprot