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Paxillin [p Tyr118] Antibody

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Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459] - Analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Pm, Rb, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Paxillin [p Tyr118] Antibody Summary

Immunogen
A portion of human Paxillin protein containing a phosphorylated tyrosine residue at position 118 was used as the immunogen for this phospho antibody.
Modification
p Tyr118
Marker
Focal Adhesion Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PXN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 30635353)
  • Western Blot 0.025-0.1 ug/ml
Publications
Read Publications using
NBP2-24459 in the following applications:

Reactivity Notes

Opossum, Chimpanzee, Orangutan, Salmon (100%), Tetradon (85%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Paxillin [p Tyr118] Antibody

  • FLJ16691
  • Paxillin
  • PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF
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Publications for Paxillin Antibody (NBP2-24459)(3)

We have publications tested in 1 confirmed species: Zebrafish.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(1)
IHC
(1)
All Applications
Filter By Species
Zebrafish
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Paxillin Antibody (NBP2-24459)

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Blogs on Paxillin.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.

CRISPR/Cas9: Keep your friends close, but your viruses closer
"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a si...  Read full blog post.

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Bioinformatics

Gene Symbol PXN
Uniprot