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Pax5/BSAP Antibody [Unconjugated]

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Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Pax5/BSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pax5/BSAP at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. ...read more
Pax5/BSAP was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human Pax5/BSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3487) at 3 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3487-SP
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Pax5/BSAP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax5/BSAP
Thr141-His391
Accession # Q02548
Specificity
Detects human Pax5/BSAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Pax2, rhPax3, and rhPax4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3487 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax5/BSAP Antibody [Unconjugated]

  • B cell specific activator protein
  • B-cell-specific transcription factor
  • BSAP
  • BSAPB-cell lineage specific activator
  • paired box 5
  • paired box gene 5 (B-cell lineage specific activator protein)
  • paired box gene 5 (B-cell lineage specific activator)
  • paired box homeotic gene 5
  • paired box protein Pax-5
  • Pax5
  • transcription factor PAX 5

Background

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 42 kDa protein belonging to the paired box transcription factor family. It is a developmental regulator that is important for B-cell lineage commitment and development. Human Pax5 is a 391 amino acid (aa) protein containing the paired DNA-binding domain at the N‑terminal region. Several alternatively spliced isoforms with altered C‑terminal regions and possessing different transactivation properties have been described. All isoforms share the N‑terminal 235 aa with full-length Pax5. Human Pax5 shares 99%, 97% and 94% aa sequence identity with mouse, bovine and canine Pax5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pax5/BSAP Antibody [Unconjugated]
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Publications for Pax5/BSAP Antibody (AF3487) (0)

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Review for Pax5/BSAP Antibody (AF3487) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3487:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Pax5/BSAP AF3487
Enlarge 		5
reviewed by:
Natalie Erdmann
 IF Human 03/03/2016
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Summary

ApplicationImmunofluorescence
Sample TestedDaudi (Burkitt Lymphoma) and U266 (Multiple Myeloma) cell lines
SpeciesHuman
LotXPI011506A

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Immunofluorescence Pax5/BSAP AF3487
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 Natalie Erdmann
03/03/2016
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol PAX5
Uniprot