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EBF-1 Antibody [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 2 ...read more
Differential binding of transcription factors on EBV genomes in type I and type III latency.(A) ChIP-Seq tracks mapped to EBV genome in LCL (red) or Mutu I (blue) for EBF1 or RBP-j kappa as indicated. EBNA2 ChIP-Seq ...read more
Cooperative binding and colocalization of EBF1, RBP-j kappa , and EBNA2 at DNA regulatory elements.(A) DNA-affinity binding assays with nuclear extracts from EREB2.5 cells with (+) or without (-) estradiol (E2) using ...read more
EBNA2-dependent transcription factor redistribution in chromatin occupancy.EREB2.5 cells were treated with (+) or without (-) estradiol (E2) for 24 or 48 hrs and then assayed by ChIP for binding to EBF1 (A and B), RBP-j ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5165-SP
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EBF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse EBF-1
Arg416-Ser520
Accession # Q07802
Specificity
Detects endogenous human and mouse EBF-1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EBF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF5165 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-1 Antibody [Unconjugated]

  • COE1
  • Collier, Olf and EBF transcription factor 1
  • early B-cell factor 1
  • Early B-cell factor
  • EBF1
  • EBF-1
  • EBFFLJ41763
  • O/E-1
  • OE-1
  • OLF1
  • OLF1FLJ39389
  • olfactory neuronal transcription factor 1
  • transcription factor COE1

Background

EBF-1 (Early B cell Factor 1; also OLF1 and COE1) is a 65-70 kDa member of the COE family of transcription factors. Although expressed in adipocytes and neurons, it is best studied in B cells where IL-7 acts to promote EBF-1 in pre-proB cells, leading to proB stage development. Mouse EBF-1 is 591 amino acids (aa) in length. It contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 370‑430, and a Pro/Ser-rich transactivation domain (aa 462‑550). EBF-1 either homodimerizes, or heterodimerizes with EBF-2 and -3. There is an alternate start site at Met134, and an isoform that shows a one aa substitution for aa 252‑259. Over aa 416‑520, mouse EBF-1 shows absolute aa identity to the equivalent sequence in rat and human EBF-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EBF-1 Antibody
[Unconjugated]
AF5165
EBF-1 Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB

Publications for EBF-1 Antibody (AF5165)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol EBF1
Uniprot