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Pancreatic Polypeptide/PP Antibody (548416) [Unconjugated]

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Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Pancreatic Polypeptide/ PP Monoclonal Antibody (Catalog # MAB62971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Pancreatic Polypeptide/ PP Monoclonal Antibody (Catalog # MAB62971) at 15 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
548416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB62971-SP
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Pancreatic Polypeptide/PP Antibody (548416) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298
Specificity
Detects human Pancreatic Polypeptide/PP in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPY
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using MAB62971.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pancreatic Polypeptide/PP Antibody (548416) [Unconjugated]

  • INN
  • Obinepitide
  • pancreatic polypeptide (ppy)
  • pancreatic polypeptide PNPPP
  • pancreatic polypeptide Y
  • Pancreatic Polypeptide
  • pancreatic prohormone
  • PNP
  • PP
  • PPY

Background

Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence (1). PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake (2). The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

  1. Leiter A, et al. (1984) J. Biol. Chem. 259:14702.
  2. Asakawa, A et al. (2003) Gastroenterology 124:1325.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pancreatic Polypeptide/PP Antibody (MAB62971)(3)


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Bioinformatics

Gene Symbol PPY
Uniprot