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p73 Antibody (5B429) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready
Clone
5B429
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

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p73 Antibody (5B429) [DyLight 680] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Specificity
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for p73 Antibody (5B429) [DyLight 680]

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Background

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p73 Antibody (NBP2-24737FR) (0)

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Secondary Antibodies

 

Isotype Controls

Additional p73 Products

Research Areas for p73 Antibody (NBP2-24737FR)

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Bioinformatics

Gene Symbol TP73