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![Western Blot: p73 Antibody (5B429) [NBP2-24737] - Consequences of DHX9 knockdown on protein levels of p53 targets in HCT116 p53+/+ and p53-/- cells. Western blot analysis of p53 transcriptional targets in HCT116 p53+/+ and p53-/- cells. Cells were harvested 6 days post-transduction and extracts were fractionated on 8% SDS-PAGE gels. All probings were performed on the same blot. Actin and eEF2 are used as loading controls. Quantitation of intensity levels of the proteins in the shDHX9 samples relative to the shFLuc. 1309 samples are indicated beneath each band. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/15889/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Western-Blot-NBP2-24737-img0009.jpg "Western Blot: p73 Antibody (5B429) [NBP2-24737] - Consequences of DHX9 knockdown on protein levels of p53 targets in HCT116 p53+/+ and p53-/- cells. Western blot analysis of p53 transcriptional targets in HCT116 p53+/+ and p53-/- cells. Cells were harvested 6 days post-transduction and extracts were fractionated on 8% SDS-PAGE gels. All probings were performed on the same blot. Actin and eEF2 are used as loading controls. Quantitation of intensity levels of the proteins in the shDHX9 samples relative to the shFLuc. 1309 samples are indicated beneath each band. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/15889/text/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: p73 Antibody (5B429) [NBP2-24737] - p73 was detected in immersion fixed Hela human cell line using p73 [5B429] Monoclonal Antibody (Catalog # NBP2-24737) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and in the nuclei.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Immunocytochemistry-Immunofluorescence-NBP2-24737-img0008.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (5B429) [NBP2-24737] - FFPE breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Immunohistochemistry-Paraffin-NBP2-24737-img0004.jpg)
![Flow Cytometry: p73 Antibody (5B429) [NBP2-24737] - Detection of p73 in Human HeLa Cell Line. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Flow-Cytometry-NBP2-24737-img0007.jpg)
![Western Blot: p73 Antibody (5B429) [NBP2-24737] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Western-Blot-NBP2-24737-img0003.jpg)
![Flow (Intracellular): p73 Antibody (5B429) [NBP2-24737] - An intracellular stain was performed on HeLa cells with pan p73 (5B429) antibody NBP2-24737 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Flow-Intracellular-NBP2-24737-img0006.jpg)
![Simple Western: p73 Antibody (5B429) [NBP2-24737] - Lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/p73-Antibody-5B429-Simple-Western-NBP2-24737-img0005.jpg)
![Western Blot: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) analysis for Np73 using NBP2-24873 at 1 ug/ml in A) a cell line transfected with Np73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-38C674-2-Western-Blot-NBP2-24873-img0010.jpg "Western Blot: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) analysis for Np73 using NBP2-24873 at 1 ug/ml in A) a cell line transfected with Np73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Delta Np73 Antibody (38C674.2) [NBP2-24873] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Delta Np73 (38C674.2) NBP2-24873 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-38C674-2-Immunocytochemistry-Immunofluorescence-NBP2-24873-img0011.jpg)
![Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-38C674-2-Immunohistochemistry-Paraffin-NBP2-24873-img0009.jpg)
![Immunohistochemistry-Frozen: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) analysis of Delta Np73 in embryonic day 15 (A) and day 17 (B) E15 rat embryonic kidney at 1:2000. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the glomerular cleft. DeltaNp73 is expressed in the ureteric bud (UB) branches, metanephrogenic mesenchyme (MM), and nephron progenitors.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-38C674-2-Immunohistochemistry-Frozen-NBP2-24873-img0007.jpg)
![Immunohistochemistry-Paraffin: Delta Np73 Antibody (38C674.2) [NBP2-24873] - Delta Np73 Antibody (38C674.2) Formalin-fixed, paraffin-embedded lung squamous cell carcinoma probed with p73 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-38C674-2-Immunohistochemistry-Paraffin-NBP2-24873-img0008.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Immunohistochemistry-Paraffin-NB100-56674-img0008.jpg "Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.")
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of the expression level of p73 isoforms. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/11774) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0010.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (5B1288) [NB100-56674] - Human breast cancer section using 10 ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Immunohistochemistry-Paraffin-NB100-56674-img0009.jpg)
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0004.jpg)
![Western Blot: p73 Antibody (5B1288) [NB100-56674] - Analysis of p73 in HeLa cell lysate (Cat no. NBP2-25045) using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/p73-Antibody-5B1288-Western-Blot-NB100-56674-img0006.jpg)
![Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NB100-420-img0003.jpg "Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: p73 Antibody [NB100-420] - Detection of Human p73 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-p73 antibody NB100-420 used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NB100-420-img0002.jpg)
![Western Blot: p73 Antibody (ER-15) [NB600-1279] - Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using p73 alpha/beta Monoclonal Antibody, shows increased levels of p73 alpha/beta in MCF7 cell line upon doxorubicin treatment.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Western-Blot-NB600-1279-img0003.jpg "Western Blot: p73 Antibody (ER-15) [NB600-1279] - Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using p73 alpha/beta Monoclonal Antibody, shows increased levels of p73 alpha/beta in MCF7 cell line upon doxorubicin treatment.")
![Western Blot: p73 Antibody (ER-15) [NB600-1279] - WB analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1) and MCF7 treated with Doxorubicine (0.5 uM Doxorubicin for 24 hours) (Lane 2). The blot was probed with Anti-p73 alpha/beta Mouse Monoclonal Antibody.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Western-Blot-NB600-1279-img0001.jpg)
![Immunofluorescence: p73 Antibody (ER-15) [NB600-1279] - Analysis of p73 alpha/beta was performed using 70% confluent log phase MCF-7 cells.](http://images.novusbio.com/fullsize/p73-Antibody-ER-15-Immunofluorescence-NB600-1279-img0002.jpg)
![Western Blot: p73 Antibody [NBP3-35073] - Western blot analysis of lysates from 293T cells, using p73 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35073_rabbit-p73-pab-10122024121055.jpg "Western Blot: p73 Antibody [NBP3-35073] - Western blot analysis of lysates from 293T cells, using p73 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: p73 Antibody [NBP3-35073] - Immunofluorescence analysis of paraffin-embedded mouse skin using p73 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35073_rabbit-p73-pab-1012202411552894.jpg)
![Immunohistochemistry: p73 Antibody [NBP3-35073] - Immunohistochemistry analysis of paraffin-embedded Human lymph node using p73 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35073_rabbit-p73-pab-101220241155281.jpg)
![Immunohistochemistry: p73 Antibody [NBP3-35073] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using p73 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35073_rabbit-p73-pab-101220241224192.jpg)
![Western Blot: p73 Antibody (7L1V7) [NBP3-16142] - Western blot analysis of extracts of various cell lines, using p73 antibody (NBP3-16142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p73-Antibody-7L1V7-Western-Blot-NBP3-16142-img0004.jpg "Western Blot: p73 Antibody (7L1V7) [NBP3-16142] - Western blot analysis of extracts of various cell lines, using p73 antibody (NBP3-16142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: p73 Antibody (7L1V7) [NBP3-16142] - Immunohistochemistry of paraffin-embedded rat brain using p73 Rabbit mAb (NBP3-16142) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p73-Antibody-7L1V7-Immunohistochemistry-Paraffin-NBP3-16142-img0003.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (7L1V7) [NBP3-16142] - Immunohistochemistry of paraffin-embedded human esophageal using p73 Rabbit mAb (NBP3-16142) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p73-Antibody-7L1V7-Immunohistochemistry-Paraffin-NBP3-16142-img0001.jpg)
![Immunohistochemistry-Paraffin: p73 Antibody (7L1V7) [NBP3-16142] - Immunohistochemistry of paraffin-embedded mouse lung using p73 Rabbit mAb (NBP3-16142) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p73-Antibody-7L1V7-Immunohistochemistry-Paraffin-NBP3-16142-img0002.jpg)
![Western Blot: p73 Antibody [NBP2-58335] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NBP2-58335-img0002.jpg "Western Blot: p73 Antibody [NBP2-58335] - Western blot analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: p73 Antibody [NBP2-58335] - Staining of human cell line HEK 293 shows localization to nucleoplasm, cell junctions & vesicles.](http://images.novusbio.com/fullsize/p73-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58335-img0001.jpg)
![Western Blot: p73 Antibody [NBP2-58523] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/p73-Antibody-Western-Blot-NBP2-58523-img0002.jpg "Western Blot: p73 Antibody [NBP2-58523] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: p73 Antibody [NBP2-58523] - Staining of human cell line HEK 293 shows localization to nucleus & the Golgi apparatus.](http://images.novusbio.com/fullsize/p73-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58523-img0001.jpg)
![Western Blot: Delta Np73 Antibody (DNp73-1.1) [NBP3-11702] - Analysis of p73 isoforms using mouse monoclonal antibody DNp73-1.1. This antibody is suitable for distinguishing between them. It detected specifically deltaNp73 isoform in H1299/deltaNp73 transfectants, whereas it ignored TAp73 isoform in H1299/TAp73 transfectants.](http://images.novusbio.com/fullsize/Delta-Np73-Antibody-DNp73-1-1-Western-Blot-NBP3-11702-img0001.jpg "Western Blot: Delta Np73 Antibody (DNp73-1.1) [NBP3-11702] - Analysis of p73 isoforms using mouse monoclonal antibody DNp73-1.1. This antibody is suitable for distinguishing between them. It detected specifically deltaNp73 isoform in H1299/deltaNp73 transfectants, whereas it ignored TAp73 isoform in H1299/TAp73 transfectants.")
![SDS-Page: TP73 Recombinant Protein [H00007161-P01] - Analysis in12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/TP73-Recombinant-Protein-SDS-Page-H00007161-P01-img0001.jpg "SDS-Page: TP73 Recombinant Protein [H00007161-P01] - Analysis in12.5% SDS-PAGE Stained with Coomassie Blue")
![Western Blot: p73 Antibody - C-terminus [NBP3-30843] - Western blot analysis using p73 alpha antibody on H1299 cells transfected with p73 alpha protein.](http://images.novusbio.com/fullsize/nbp3-30843_rabbit-p73-pab-c-terminus-811202420592125.jpg "Western Blot: p73 Antibody - C-terminus [NBP3-30843] - Western blot analysis using p73 alpha antibody on H1299 cells transfected with p73 alpha protein.")