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P-Selectin/CD62P Antibody (9E1) [Unconjugated]

 (9 Publications)
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P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
P-Selectin/CD62P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human P-Selectin/CD62P Monoclonal Antibody (Catalog # BBA30) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N
Clone
9E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Fluorescein
BBA34
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Carrier Free
BBA34
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P-Selectin/CD62P Antibody (9E1) [Unconjugated] Summary

Immunogen
Recombinant human P-Selectin
Extracellular domain
Specificity
This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
BBA30 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Selectin/CD62P Antibody (9E1) [Unconjugated]

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

P-Selectin, also known as GMP-140 and PADGEM, is a transmembrane glycoprotein expressed by activated platelets and endothelial cells. P-Selectin binds to PSGL-1 on myeloid cells, neutrophils, monocytes, and lymphocytes. P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Selectin/CD62P Antibody (BBA30)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-Fr, Neutralization.


Filter By Application
IHC
(1)
IHC-Fr
(1)
Neutralization
(3)
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Human
(5)
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Showing Publications 1 - 9 of 9.
Publications using BBA30 Applications Species
Eugenio D. Hottz, Remy Martins-Gonçalves, Lohanna Palhinha, Isaclaudia G. Azevedo-Quintanilha, Mariana M. de Campos, Carolina Q. Sacramento et al. Platelet-monocyte interaction amplifies thromboinflammation through tissue factor signaling in COVID-19 Blood Advances 2022-09-13 [PMID: 35420680]
Luca Perico, Marina Morigi, Miriam Galbusera, Anna Pezzotta, Sara Gastoldi, Barbara Imberti et al. SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation Frontiers in Immunology 2022-03-07 [PMID: 35320941]
Li T, Yang Y, Li Y et al. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein The Journal of clinical investigation 12/29/2021 [PMID: 34964720]
Hiten D. Mistry, Melissa V. Hott Ogalde, Fiona Broughton Pipkin, Geneviève Escher, Lesia O. Kurlak Maternal, Fetal, and Placental Selectins in Women With Pre-eclampsia; Association With the Renin-Angiotensin-System Frontiers in Medicine 2020-06-12 [PMID: 32596247]
M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events Cancer Res., 2019-10-29;0(0):. 2019-10-29 [PMID: 31662326] (IHC, Human) IHC Human
Hottz E, Medeiros-de-Moraes I, Vieira-de-Abreu A, de Assis E, Vals-de-Souza R, Castro-Faria-Neto H, Weyrich A, Zimmerman G, Bozza F, Bozza P Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue. J Immunol, 2014-07-11;193(4):1864-72. 2014-07-11 [PMID: 25015827] (Neutralization, Human) Neutralization Human
Singh , Meera V, Davidson , Donna C, Jackson , Joseph W, Singh , Vir B, Silva , Jharon, Ramirez , Servio H, Maggirwar , Sanjay B Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation. J Immunol, 2014-04-11;192(10):4674-84. 2014-04-11 [PMID: 24729609] (Neutralization, Human) Neutralization Human
Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Dev. Biol., 2006-06-15;298(1):107-17. 2006-06-15 [PMID: 16930583] (IHC-Fr, Human) IHC-Fr Human
Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin. Mol. Cells, 2005-06-30;19(3):350-5. 2005-06-30 [PMID: 15995351] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol SELP