![Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Western-Blot-NBP2-33211-img0001.jpg "Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | 98A1071 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody. |
| Specificity | The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TNFRSF11B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS - BSA free |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Osteoprotegerin/TNFRSF11B Antibody (NBP2-33211)Find related products by research area.
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| Gene Symbol | TNFRSF11B |
![Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33211-img0002.jpg "Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Staining of human liver. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Immunohistochemistry-NBP2-33211-img0006.jpg "Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Staining of human liver. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.")
![Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Flow-Cytometry-NBP2-33211-img0007.jpg "Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary. Image from the standard format of this antibody.")
![Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Immunohistochemistry-NBP2-33211-img0003.jpg "Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.")
![Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin an](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Immunohistochemistry-NBP2-33211-img0004.jpg "Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin an")
![Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Staining of human heart tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Osteoprotegerin-TNFRSF11B-Antibody-98A1071-Azide-Free-Immunohistochemistry-NBP2-33211-img0005.jpg "Immunohistochemistry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - Azide Free [NBP2-33211] - Staining of human heart tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Image from the standard format of this antibody.")
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