Are the following monoclonals purified from ascites or from tissue culture supernatant?NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

Name: Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free
Brand: Novus Biologicals
SKU: NB100-56505
Price: 399 USD

Question

Accepted Answer

These antibodies are all purified from tissue culture supernatant.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, RbSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone	98A1071
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-56505AF350

Alexa Fluor 405 NB100-56505AF405

Alexa Fluor 488 NB100-56505AF488

Alexa Fluor 532 NB100-56505AF532

Alexa Fluor 594 NB100-56505AF594

Alexa Fluor 647 NB100-56505AF647

Alexa Fluor 700 NB100-56505AF700

Alexa Fluor 750 NB100-56505AF750

Allophycocyanin NB100-56505APC

Allophycocyanin/Cy7 NB100-56505APCCY7

Biotin NB100-56505B

CoraFluor 1 NB100-56505CL1

DyLight 350 NB100-56505UV

DyLight 405 NB100-56505V

DyLight 488 NB100-56505G

DyLight 550 NB100-56505R

DyLight 594 NB100-56505DL594

DyLight 650 NB100-56505C

DyLight 680 NB100-56505FR

DyLight 755 NB100-56505IR

FITC NB100-56505F

HRP NB100-56505H

Janelia Fluor 525 NB100-56505JF525

Janelia Fluor 549 NB100-56505JF549

Janelia Fluor 585 NB100-56505JF585

Janelia Fluor 635 NB100-56505JF635

Janelia Fluor 646 NB100-56505JF646

Janelia Fluor 669 NB100-56505JF669

PE NB100-56505PE

PE/Atto594 NB100-56505PEATT594

PE/Cy5.5 NB100-56505PECY55

PE/Cy7 NB100-56505PECY7

PerCP NB100-56505PCP

mFluor Violet 450 SE NB100-56505MFV450

mFluor Violet 500 SE NB100-56505MFV500

mFluor Violet 610 SE NB100-56505MFV610

Catalog# & Formulation	Size	Price
BSA Free NB100-56505SS

Azide Free NBP2-33211

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56505AF350
Alexa Fluor 405	NB100-56505AF405
Alexa Fluor 488	NB100-56505AF488
Alexa Fluor 532	NB100-56505AF532
Alexa Fluor 594	NB100-56505AF594
Alexa Fluor 647	NB100-56505AF647
Alexa Fluor 700	NB100-56505AF700
Alexa Fluor 750	NB100-56505AF750
Allophycocyanin	NB100-56505APC
Allophycocyanin/Cy7	NB100-56505APCCY7
Biotin	NB100-56505B
CoraFluor 1	NB100-56505CL1
DyLight 350	NB100-56505UV
DyLight 405	NB100-56505V
DyLight 488	NB100-56505G
DyLight 550	NB100-56505R
DyLight 594	NB100-56505DL594
DyLight 650	NB100-56505C
DyLight 680	NB100-56505FR
DyLight 755	NB100-56505IR
FITC	NB100-56505F
HRP	NB100-56505H
Janelia Fluor 525	NB100-56505JF525
Janelia Fluor 549	NB100-56505JF549
Janelia Fluor 585	NB100-56505JF585
Janelia Fluor 635	NB100-56505JF635
Janelia Fluor 646	NB100-56505JF646
Janelia Fluor 669	NB100-56505JF669
PE	NB100-56505PE
PE/Atto594	NB100-56505PEATT594
PE/Cy5.5	NB100-56505PECY55
PE/Cy7	NB100-56505PECY7
PerCP	NB100-56505PCP
mFluor Violet 450 SE	NB100-56505MFV450
mFluor Violet 500 SE	NB100-56505MFV500
mFluor Violet 610 SE	NB100-56505MFV610

Osteoprotegerin/TNFRSF11B Antibody (98A1071) - BSA Free

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Publications for Osteoprotegerin/TNFRSF11B Antibody (NB100-56505)(44)

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Immunogen	A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody.
Specificity	The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	TNFRSF11B
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NB100-56505	Applications	Species
Zhang W, Yu L, Wang F et al. Rosavin regulates bone homeostasis through HDAC1-induced epigenetic regulation of EEF2 Chemico-biological interactions 2023-09-09 [PMID: 37689331] (IHC, Mouse)	IHC	Mouse
Hild V, Mellert K, Möller P, Barth TFE Giant Cells of Various Lesions Are Characterised by Different Expression Patterns of HLA-Molecules and Molecules Involved in the Cell Cycle, Bone Metabolism, and Lineage Affiliation: An Immunohistochemical Study with a Review of the Literature Cancers 2023-07-21 [PMID: 37509363] (IHC-P, Human) Details: 1:400 IHC-P dilution	IHC-P	Human
Kumar K, Datta K, Fornace A, Suman S Total body proton and heavy-ion irradiation causes cellular senescence and promotes pro-osteoclastogenic activity in mouse bone marrow Heliyon 2022-01-01 [PMID: 35028468] (IHC-P, Mouse)	IHC-P	Mouse
Lysitska A, Galanis N, Skandalos I Et al. Histology and Immunohistochemistry of Radial Arteries Are Suggestive of an Interaction between Calcification and Early Atherosclerotic Lesions in Chronic Kidney Disease Medicina 2021-10-24 [PMID: 34833374] (IHC-P, Human)	IHC-P	Human
Zhang Q, Chen T, Zhang Y, et al. MiR-30c-5p regulates adventitial progenitor cells differentiation to vascular smooth muscle cells through targeting OPG Stem Cell Research & Therapy 2021-12-01 [PMID: 33468212] (WB)	WB
Jiang Y, Li J, Li G et al. Osteoprotegerin interacts with syndecan-1 to promote human endometrial stromal decidualization by decreasing Akt phosphorylation Hum Reprod 2020-11-01 [PMID: 33047116]
Miyata T, Minami M, Kataoka H et al. Osteoprotegerin Prevents Intracranial Aneurysm Progression by Promoting Collagen Biosynthesis and Vascular Smooth Muscle Cell Proliferation Journal of the American Heart Association 2020-09-01 [PMID: 32856519]
Bayer Alinca S, Saglam E, Zengin Celik T et al. Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1alpha, RANKL and OPG Biotech Histochem 2020-04-15 [PMID: 32292090]
Sengun MC, Gunpinar S Effects of systemic hydroxytyrosol application in experimental periodontitis of rats International Journal of Plant Based PHARMACEUTICALS 2022-01-01 (IF/IHC, Rat)	IF/IHC	Rat
Lee YC, Chan YH, Hsieh SC et al. Comparing the Osteogenic Potentials and Bone Regeneration Capacities of Bone Marrow and Dental Pulp Mesenchymal Stem Cells in a Rabbit Calvarial Bone Defect Model Int J Mol Sci 2019-10-10 [PMID: 31658685] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Wang X, Cao Y, Liu Z et al. Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction Balkan Med J 2019-10-28 [PMID: 31290639] (WB, Rat)	WB	Rat
Gegen T, Zhu Y, Sun QHou B Role of interleukin-33 in the clinical pathogenesis of chronic apical periodontitis J. Int. Med. Res. 2019-06-20 [PMID: 31218936] (IF/IHC, Human)	IF/IHC	Human
Li , Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis 2018-08-12 [PMID: 30082761] (WB, IF/IHC, Human)	WB, IF/IHC	Human
El Kholy K, Freire M, Chen T, Van Dyke TE. Resolvin E1 Promotes Bone Preservation Under Inflammatory Conditions Front Immunol 2018-06-12 [PMID: 29946319] (Mouse)		Mouse
Nozawa S, Inubushi T, Irie F, Takigami I. Osteoblastic heparan sulfate regulates osteoprotegerin function and bone mass. JCI Insight. 2018-02-08 [PMID: 29415886] (ICC/IF, Human)	ICC/IF	Human
Scimeca M, Salustri A, Bonanno E et al. Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis. 2017-10-12 [PMID: 29022895] (Human)		Human
Stanislawowski M, Wisniewski P, Guzek M et al. Influence of receptor activator of nuclear factor kappa B ligand, osteoprotegerin and interleukin-33 on bone metabolism in patients with long-standing ulcerative colitis. J Crohns Colitis. 2014-01-15 [PMID: 24439762] (IHC-Fr, Human) Details: Colonic mucosa biopsies, Fig 7C	IHC-Fr	Human
Larson SR, Zhang X, Dumpit R et al. Characterization of osteoblastic and osteolytic proteins in prostate cancer bone metastases. Prostate. 2013-06-01 [PMID: 23334979]
Boumans MJ, Thurlings RM, Yeo L et al. Rituximab abrogates joint destruction in rheumatoid arthritis by inhibiting osteoclastogenesis. Ann Rheum Dis. 2012-01-01 [PMID: 22072013]
Witasp Anna, Carrero Juan J, Hammarqvist Folke et al. Expression of osteoprotegerin in human fat tissue; implications for chronic kidney disease. Eur J Clin Invest. 2011-05-01 [PMID: 21128937] (Human)		Human
Tomlins Christine, Storey Alan. Cutaneous HPV5 E6 causes increased expression of Osteoprotegerin and Interleukin 6 which contribute to evasion of UV-induced apoptosis. Carcinogenesis. 2010-12-01 [PMID: 20884685] (WB)	WB
Vandooren B, Cantaert T, Noordenbos T et al. The abundant synovial expression of the RANK/RANKL/Osteoprotegerin system in peripheral spondylarthritis is partially disconnected from inflammation. Arthritis Rheum. 2008-03-01 [PMID: 18311801]
Waterman EA, Cross NA, Lippitt JM et al. The antibody MAB8051 directed against osteoprotegerin detects carbonic anhydrase II: implications for association studies with human cancers. Int J Cancer. 2007-11-01 [PMID: 17631639]
Pettit AR, Walsh NC, Manning C et al. RANKL protein is expressed at the pannus-bone interface at sites of articular bone erosion in rheumatoid arthritis. Rheumatology (Oxford). 2006-09-01 [PMID: 16490750]
Sandra F, Hendarmin L, Nakamura S. Osteoprotegerin (OPG) binds with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): suppression of TRAIL-induced apoptosis in ameloblastomas. Oral Oncol. 2006-04-01 [PMID: 16413220]
Pettersen Ingvild, Bakkelund Wenche, Smedsrod Bard, Sveinbjornsson Baldur. Osteoprotegerin is expressed in colon carcinoma cells. Anticancer Res. 2005-11-01 [PMID: 16309167]
Gaudio A, Lasco A, Morabito N et al. Hepatic osteodystrophy: does the osteoprotegerin/receptor activator of nuclear factor-kB ligand system play a role? J Endocrinol Invest. 2005-09-01 [PMID: 16277162] (IHC-P)	IHC-P
Kresnoadi U, Laksono V, Dahlan A Expression and ratio of receptor activator of nuclear factor kappa-B ligand and osteoprotegerin following application of Nigella sativa/bovine bone graft combination in post tooth extraction sockets The Journal of Indian Prosthodontic Society 2023-07-14 (IHC-P, Guinea Pig)	IHC-P	Guinea Pig
Kresnoadi U, Sari N, Laksono H Socket preservation using a combination of propolis extract and bovine bone graft towards the expression of receptor activator of nuclear ?B ligand and osteoprogerin Folia Medica 2023-10-31 (IHC, Guinea Pig)	IHC	Guinea Pig
Pohjolainen V, Taskinen P, Soini Y et al. Noncollagenous bone matrix proteins as a part of calcific aortic valve disease regulation. Hum Pathol. 2008-11-01 [PMID: 18701137]
Song A, Wang W, Zhang Y et al. Acetylsalicylic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Mesenchymal Stem Cells and Regeneration of Alveolar Bone in Experimental Periodontitis Rats Journal of Tissue Engineering and Regenerative Medicine 2023-11-03 (IHC-P, Rat) Details: Dilution 1:50, Tissue type: axillas	IHC-P	Rat
Moschen AR, Kaser A, Stadlmann S et al. The RANKL/OPG system and bone mineral density in patients with chronic liver disease. J Hepatol. 2005-12-01 [PMID: 16143421]
Moran CS, McCann M, Karan M et al. Association of osteoprotegerin with human abdominal aortic aneurysm progression. Circulation. 2005-06-14 [PMID: 15939823] (IHC-P, WB) Details: IHC (p): healthy and aneurysmal aortic biopsies, Fig 2A,B. WB: aortic biopsies (Fig 2C, D) and recombinant OPG (Fig 2D). OPG was detected at 55 kDa. An ~75 kDa band was also detected on one of the samples. OPG quantification was performed by comparison of	IHC-P, WB
Sandra F, Hendarmin L, Kukita T et al. Ameloblastoma induces osteoclastogenesis: a possible role of ameloblastoma in expanding in the bone. Oral Oncol. 2005-07-01 [PMID: 15935726] (ELISA, Human) Details: Ameloblastoma tumor tissue and AM-1 cells: 1. OPG (IMG-103A), ELISA (detection): Results described.	ELISA	Human
Moschen AR, Kaser A, Enrich B et al. The RANKL/OPG system is activated in inflammatory bowel disease and relates to the state of bone loss. Gut. 2005-04-01 [PMID: 15753532] (IHC-P)	IHC-P
Vidal K, Serrant P, Schlosser B et al. Osteoprotegerin production by human intestinal epithelial cells: a potential regulator of mucosal immune responses. Am J Physiol Gastrointest Liver Physiol. 2004-10-01 [PMID: 15521102]
Vidal K, van den Broek P, Lorget F et al. Osteoprotegerin in human milk: a potential role in the regulation of bone metabolism and immune development. Pediatr Res. 2004-06-01 [PMID: 15155868]
Golledge J, McCann M, Mangan S, Lam A et al. Osteoprotegerin and osteopontin are expressed at high concentrations within symptomatic carotid atherosclerosis. Stroke. 2004-07-01 [PMID: 15143295]
Standal T, Seidel C, Hjertner O et al. Osteoprotegerin is bound, internalized, and degraded by multiple myeloma cells. Blood. 2002-10-15 [PMID: 12351414] (IHC-P)	IHC-P
Komuro H, Olee T, Kuhn K et al. The osteoprotegerin/receptor activator of nuclear factor kappaB/receptor activator of nuclear factor kappaB ligand system in cartilage. Arthritis Rheum. 2001-12-01 [PMID: 11762937]
Fiumara P, Snell V, Li Y et al. Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines. Blood. 2001-11-01 [PMID: 11675352]
Pearse RN, Sordillo EM, Yaccoby S et al. Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression. Proc Natl Acad Sci U S A. 2001-09-25 [PMID: 11562486] Details: Antibodies cited [IHC (paraffin)], Figs 1 and 4 (human plasmocytoma and decalified bone marrow): 1. OPG (IMG-103) 2. Trance/RankL/OPGL/ODF (IMG-133).
Saika M, Inoue D, Kido S, Matsumoto T. 17beta-estradiol stimulates expression of osteoprotegerin by a mouse stromal cell line, ST-2, via estrogen receptor-alpha. Endocrinology. 2001-06-01 [PMID: 11356664]
Kobayashi Y, Hashimoto F, Miyamoto H et al. Force-induced osteoclast apoptosis in vivo is accompanied by elevation in transforming growth factor beta and osteoprotegerin expression. J Bone Miner Res. 2000-10-01 [PMID: 11028444] (Rat)		Rat
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Gene Symbol	TNFRSF11B
Entrez	690(Human)
Uniprot	O00300()