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Osteopontin/OPN Antibody (1B20) [Janelia Fluor® 525]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP
Clone
1B20
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-89062AF350
Alexa Fluor 405
NB110-89062AF405
Alexa Fluor 488
NB110-89062AF488
Alexa Fluor 532
NB110-89062AF532
Alexa Fluor 594
NB110-89062AF594
Alexa Fluor 647
NB110-89062AF647
Alexa Fluor 700
NB110-89062AF700
Alexa Fluor 750
NB110-89062AF750
Biotin
NB110-89062B
CoraFluor 1
NB110-89062CL1
DyLight 350
NB110-89062UV
DyLight 405
NB110-89062V
DyLight 488
NB110-89062G
DyLight 550
NB110-89062R
DyLight 594
NB110-89062DL594
DyLight 650
NB110-89062C
DyLight 680
NB110-89062FR
DyLight 755
NB110-89062IR
FITC
NB110-89062F
HRP
NB110-89062H
Janelia Fluor 549
NB110-89062JF549
Janelia Fluor 646
NB110-89062JF646
Janelia Fluor 669
NB110-89062JF669
Unconjugated
NB110-89062
mFluor Violet 450 SE
NB110-89062MFV450
mFluor Violet 500 SE
NB110-89062MFV500
mFluor Violet 610 SE
NB110-89062MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB110-89062
BSA Free
NB110-89062SS
BSA Free
NB110-89062
Azide and BSA Free
NBP2-80895
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Osteopontin/OPN Antibody (1B20) [Janelia Fluor® 525] Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, Rat and Rabbit. Rabbit reactivity reported in scientific literature (PMID:33001866). Use in Mouse reported in scientific literature (PMID:33580126).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Osteopontin/OPN Antibody (1B20) [Janelia Fluor® 525]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL27/CTACK [HRP]
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N/A CCL27/CTACK [HRP]
N/A CCL27/CTACK [HRP]
(10 Publications)
Species: Hu
Applications: ELISA
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Applications: WB
ASRGL1 Antibody
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NBP3-25358
Immunohistochemistry-Paraffin PDLIM3 Antibody (HL2075) - Azide and BSA Free
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Western Blot PDLIM3 Antibody (HL2075) - Azide and BSA Free
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Osteoprotegerin/TNFRSF
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Azide and BSA Free
NBP2-92546
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M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(663 Publications)
Species: Mu
Applications: ELISA
Osteopontin/OPN
Antibody (1B20) [Jane ...
NB110-89062JF525
Osteopontin/OPN Antibody (1B20) [Janelia Fluor® 525]
Species: Hu, Mu, Rt, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPP1