![Immunohistochemistry-Paraffin: Orc2 Antibody [NBP1-46175] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunohistochemistry-Paraffin-NBP1-46175-img0014.jpg "Immunohistochemistry-Paraffin: Orc2 Antibody [NBP1-46175] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 1 and 50 of human Origin Recognition Complex, Subunit 2-like using the numbering given in entry NP_006181.1 (GeneID 4999). |
| Marker | Chromatin Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ORC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-46175 | Applications | Species |
|---|---|---|
| Murai J, Tang SW, Leo E, Baechler SA. SLFN11 Blocks Stressed Replication Forks Independently of ATR. Mol. Cell. 2018-02-01 [PMID: 29395061] (Human) | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Orc2 Antibody (NBP1-46175)Find related products by research area.
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| Gene Symbol | ORC2 |
![Immunoprecipitation: Orc2 Antibody [NBP1-46175] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46175 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46176, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunoprecipitation-NBP1-46175-img0015.jpg "Immunoprecipitation: Orc2 Antibody [NBP1-46175] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46175 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46176, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")