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ORC3L Antibody

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Western Blot: ORC3L Antibody [NB100-796] - Staining of HeLa lysate (RIPA buffer, 1.4E5 cells per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Western Blot: ORC3L Antibody [NB100-796] - Staining of HeLa lysate (RIPA buffer, 1.4E5 cells per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORC3L Antibody [NB100-796] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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ORC3L Antibody Summary

Immunogen
Peptide with sequence QKTDHVARLTWGGC corresponding to C-Terminus according to NP_862820.1, NP_036513.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ORC3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 75 kDa band observed in lysates of HeLa (calculated MW of 82.3 kDa band according to NP_862820.1 and NP_036513.2). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC-P: Paraffin embedded Human Brain (Cortex)
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-796.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ORC3L Antibody

  • IMAGE50150
  • LAT
  • LATHEOorigin recognition complex, subunit 3-like (yeast)
  • ORC3Lhomolog of latheo, Drosophila
  • origin recognition complex subunit 3
  • Origin recognition complex subunit Latheo
  • origin recognition complex, subunit 3 (yeast homolog)-like
  • origin recognition complex, subunit 3 honolog (yeast)
  • origin recognition complex, subunit 3 honolog
  • origin recognition complex, subunit 3
  • origin recognition complex, subunit 3-like

Background

ORC3L is involved in DNA replication origin binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ORC3L Antibody (NB100-796)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ORC3
Entrez
Uniprot