![Western Blot: Cdc6 Antibody [NBP2-15837] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cdc6-Antibody-Western-Blot-NBP2-15837-img0005.jpg "Western Blot: Cdc6 Antibody [NBP2-15837] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cdc6. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDC6 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-15837 | Applications | Species |
|---|---|---|
| White JR, Thompson DT, Koch KE et al. AP-2?-Mediated Activation of E2F and EZH2 Drives Melanoma Metastasis Cancer Research 2021-09-01 [PMID: 34210752] (Western Blot) | Western Blot |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cdc6 Antibody (NBP2-15837)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Cdc6 Antibody [NBP2-15837] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC6 protein stained by CDC6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cdc6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15837-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cdc6 Antibody [NBP2-15837] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC6 protein stained by CDC6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Cdc6 Antibody [NBP2-15837] - Human oral carcinoma. CDC6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cdc6-Antibody-Immunohistochemistry-Paraffin-NBP2-15837-img0007.jpg "Immunohistochemistry-Paraffin: Cdc6 Antibody [NBP2-15837] - Human oral carcinoma. CDC6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264. 7 whole cell lysate/ extract 7.5% SDS Page Gel, antibody diluted 1:1000](http://images.novusbio.com/fullsize/Cdc6-Antibody-Western-Blot-NBP2-15837-img0001.jpg "Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264. 7 whole cell lysate/ extract 7.5% SDS Page Gel, antibody diluted 1:1000")
![Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGECdc6 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Cdc6-Antibody-Western-Blot-NBP2-15837-img0002.jpg "Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGECdc6 antibody dilution: 1:1000.")
![Western Blot: Cdc6 Antibody [NBP2-15837] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cdc6-Antibody-Western-Blot-NBP2-15837-img0003.jpg "Western Blot: Cdc6 Antibody [NBP2-15837] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody diluted at 1:500.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")