![Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Western-Blot-NBP2-19601-img0002.jpg "Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary. |
| Localization | Golgi apparatus; cis-Golgi network membrane; Peripheral membrane protein; Lumenal side (By similarity); Cytoplasm (By similarity) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NUCB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Simple Western CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Simple_Western_17480.jpg)
![Western Blot CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Western_Blot_18637.jpg)
![Immunohistochemistry CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/SCF_R_AF1356_Immunohistochemistry_9746.jpg)
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Nucleobindin 1 antibody detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody diluted at 1:20000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19601-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Nucleobindin 1 antibody detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody diluted at 1:20000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19601-img0007.jpg "Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Western-Blot-NBP2-19601-img0001.jpg "Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19601-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Nucleobindin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-19601-img0005.jpg "Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Western Blot XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Western_Blot_5911.jpg)
![Simple Western XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Simple_Western_16640.jpg)
![Knockout Validated XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Knockout_Validated_22488.jpg)
![Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]](https://images.novusbio.com/images2/TFF2_MAB4077_Immunohistochemistry_21485.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")