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EPHX2 Antibody (OTI1A6)

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Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] Analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human prostate tissue using anti-EPHX2 mouse monoclonal antibody.
Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human endometrium tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human Kidney tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human liver tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human lymphoma tissue using anti-EPHX2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human pancreas tissue using anti-EPHX2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.7 mg/ml

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EPHX2 Antibody (OTI1A6) Summary

Immunogen
Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPHX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-02948 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31002701). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EPHX2 Antibody (OTI1A6)

  • Bifunctional Epoxide Hydrolase 2
  • CEH
  • Cytosolic epoxide hydrolase
  • EC 3.3.2.10
  • EPHX2
  • Epoxide Hydratase 2
  • Epoxide hydratase
  • Epoxide Hydrolase 2
  • epoxide hydrolase 2, cytoplasmic
  • Epoxide Hydrolase 2, Cytosolic
  • epoxide hydrolase, soluble
  • SEH
  • Soluble epoxide hydrolase

Background

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EPHX2 Antibody (NBP2-02948)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EPHX2
Entrez