![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0005.jpg "Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | An internal synthetic peptide made to the human NSP 5 alpha 3 alpha protein sequence (between residues 1-100). [UniProt# Q5M775] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SPECC1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, a band is seen at ~80 kDa. A second band may be seen at ~60 kDa. The specificity of this band is not known. |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for NSP 5 alpha 3 alpha Antibody (NB100-517)Find related products by research area.
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![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western Blot analysis of total lysates from asynchronous HN30 cells and MCF-12a 3 days post-transfection. NT: non-treated, OB: only buffer, EGFP: only pcDNA3.1/CT-GFP vector, NSP 5a3a/EGFP: pcDNA 3.1/CT-GFP and pcDNA 3.0 NSP 5a3a. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0007.jpg "Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western Blot analysis of total lysates from asynchronous HN30 cells and MCF-12a 3 days post-transfection. NT: non-treated, OB: only buffer, EGFP: only pcDNA3.1/CT-GFP vector, NSP 5a3a/EGFP: pcDNA 3.1/CT-GFP and pcDNA 3.0 NSP 5a3a. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.")
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NB100-517-img0004.jpg "Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.")
![Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-517-img0003.jpg "Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - The NSP 5 alpha 3 antibody was tested in Hela cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.")
![Immunoprecipitation: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated. NT: non-treated HN30 cells, a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunoprecipitation-NB100-517-img0006.jpg "Immunoprecipitation: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated. NT: non-treated HN30 cells, a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a and western blot analysis using rabbit anti-TRAF2. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/306/text/) licensed under a CC-BY license.")
![Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-517_rabbit-polyclonal-nsp-5-alpha-3-alpha-antibody-310202415525234.jpg "Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western blot analysis of co-immunoprecipitations of asynchronous HN30 non-treated & HN30 cells transfected with pcDNA 3.0 NSP 5a3a & pcDNA 3.1/CT-GFP for 3 daysNT: non-treated HN30 cells, TRD: HN30 cells over-expressing NSP 5a3a. a. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-DAXX., b. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot anaylsis using rabbit anti-DAXX., c. Immunoprecipitation of non-treated HN30 cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., d. Immunoprecipitation of HN30 treated cells using rabbit anti-NSP 5a3a & western blot analysis using rabbit anti-TRAF2., e. Immunoprecipitation of non-treated HN30 cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a, & f. Immunoprecipitation of HN30 treated cells using rabbit anti-TRAF2/DAXX & western blot analysis using rabbit anti-NSP 5a3a. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")