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NOC2L Antibody

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Immunocytochemistry/Immunofluorescence: NOC2L Antibody [NBP2-56871] - Staining of human cell line MCF7 shows localization to nucleoli.
Immunocytochemistry/ Immunofluorescence: NOC2L Antibody [NBP2-56871] - Staining of human cell line MCF7 shows localization to nucleoli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NOC2L Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LLGKVQENSAYICSRRQRVSFGVSEQQAVEAWEKLTREEGTPLTLYYSHWRKLRDREIQLEISGKERLEDLNFPEIKRRKMADRKDEDRKQFKDLFDLNSS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOC2L
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NOC2L Recombinant Protein Antigen (NBP2-56871PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NOC2L Antibody

  • DKFZP564C186
  • FLJ35172
  • NET15
  • NET7
  • NIR
  • NOC2-like protein
  • novel INHAT (inhibitor of histone acetyltransferase) repressor
  • nucleolar complex associated 2 homolog (S. cerevisiae)
  • nucleolar complex protein 2 homolog
  • Protein NOC2 homolog

Background

NOC2L is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity. NOC2L or NIR (Novel INHAT Repressor) is ubiquitously expressed throughout embryonic development and adulthood. It is a potent transcriptional corepressor that is not blocked by histone deacetylase inhibitors and is capable of silencing both basal and activator-driven transcription. NOC2L directly binds to nucleosomes and core histones and prevents acetylation by histone acetyltransferases, thus acting as a bona fide INHAT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NOC2L