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NKIRAS1 Antibody - Azide and BSA Free

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Western Blot: NKIRAS1 Antibody [NBP2-93958] - Analysis of extracts of various cell lines, using NKIRAS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug ...read more
Immunocytochemistry/ Immunofluorescence: NKIRAS1 Antibody [NBP2-93958] - Immunofluorescence analysis of PC-12 cells using NKIRAS1 Rabbit pAb (NBP2-93958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: NKIRAS1 Antibody [NBP2-93958] - Immunofluorescence analysis of NIH/3T3 cells using NKIRAS1 Rabbit pAb (NBP2-93958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Catalog# & Formulation Size Price
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NKIRAS1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 118-192 of human NKIRAS1 (NP_065078.1). LGNKIDLSEQRQVDAEVAQQWAKSEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLPGRKNKGNSNSEN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NKIRAS1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for NKIRAS1 Antibody - Azide and BSA Free

  • I-kappa-B-interacting Ras-like protein 1
  • kappa B-ras 1
  • Kappa B-Ras protein 1
  • KappaB-Ras1
  • KBRAS1kappaB-Ras1
  • NF-kappa-B inhibitor-interacting Ras-like protein 1
  • NFKB inhibitor interacting Ras-like 1
  • NFKB inhibitor interacting Ras-like protein 1

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first genes induced following NF-kB activation is IkBa. Recently, two proteins, kB-Ras1 and kB-Ras2 have been identified. These two proteins interact with PEST domains of IkBa and IkBb and decrease their rate of degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKIRAS1 Antibody (NBP2-93958) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NKIRAS1