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NKG2D/CD314 Antibody (149810) [Alexa Fluor® 700]

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Human peripheral blood lymphocytes gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2D/CD314 Alexa Fluor® ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
149810
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

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NKG2D/CD314 Antibody (149810) [Alexa Fluor® 700] Summary

Additional Information
Clone 149810 was used by HLDA to establish CD designation
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLRK1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
Publications
Read Publications using
FAB139N in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Alexa Fluor® 700]

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2D/CD314 Antibody (FAB139N)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, ICC.


Filter By Application
Flow Cytometry
(4)
ICC
(1)
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Filter By Species
Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using FAB139N Applications Species
Jürgens, M;Claus, M;Wingert, S;Niemann, JA;Picard, LK;Hennes, E;Haasler, I;Hellwig, B;Overbeck, N;Reinders, J;Rahnenführer, J;Schedel, M;Capellino, S;Watzl, C; Chronic stimulation desensitizes ?2-adrenergic receptor responses in natural killer cells European journal of immunology 2024-09-30 [PMID: 39350450] (Flow Cytometry, Human) Flow Cytometry Human
Picard, LK;Niemann, JA;Littwitz-Salomon, E;Waldmann, H;Watzl, C; Restriction of Glycolysis Increases Serial Killing Capacity of Natural Killer Cells International journal of molecular sciences 2024-03-02 [PMID: 38474166] (Flow Cytometry, Human) Flow Cytometry Human
LK Picard, E Littwitz-S, H Waldmann, C Watzl Inhibition of Glucose Uptake Blocks Proliferation but Not Cytotoxic Activity of NK Cells Cells, 2022-11-03;11(21):. 2022-11-03 [PMID: 36359883] (Flow Cytometry, Human) Flow Cytometry Human
van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset. PLoS Pathog, 2015-03-24;11(3):e1004671. 2015-03-24 [PMID: 25803478] (Flow Cytometry, Human) Flow Cytometry Human
Martin-Antonio B, Najjar A, Robinson S, Chew C, Li S, Yvon E, Thomas M, Mc Niece I, Orlowski R, Munoz-Pinedo C, Bueno C, Menendez P, Fernandez de Larrea C, Urbano-Ispizua A, Shpall E, Shah N Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking. Cell Death Differ, 2014-08-29;22(1):96-107. 2014-08-29 [PMID: 25168239] (ICC, Human) ICC Human

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Secondary Antibodies

 

Isotype Controls

Additional NKG2D/CD314 Products

Blogs on NKG2D/CD314.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Bioinformatics

Gene Symbol KLRK1