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ULBP-1 Antibody (170818) [Unconjugated]

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MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP‑1 Monoclonal Antibody (Catalog # MAB1380, filled histogram) or isotype control antibody (Catalog # MAB003, open ...read more
Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
170818
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ULBP-1 Antibody (170818) [Unconjugated] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-1
Specificity
Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ULBP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB1380 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-1 Antibody (170818) [Unconjugated]

  • ALCAN-beta
  • MULT-1
  • N2DL1
  • N2DL-1
  • NKG2D ligand 1
  • NKG2DL
  • NKG2DL1
  • RAET1I
  • RAET1Ialcan-beta
  • Retinoic acid early transcript 1I
  • UL16 binding protein 1
  • UL16-binding protein 1
  • ULBP1
  • ULBP-1

Background

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ULBP-1 Antibody (MAB1380)(65)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, Functional Assay, IHC, Immunoprecipitation, Neutralization.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(45)
Functional Assay
(1)
IHC
(1)
Immunoprecipitation
(1)
Neutralization
(4)
All Applications
Filter By Species
Human
(52)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using MAB1380 Applications Species
Yi Zhang, Feifei Luo, Kuiran Dong Soluble NKG2D ligands impair CD8+ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma Oncology Letters 2023-05-24 [PMID: 37274476]
Koh, EK;Lee, HR;Son, WC;Park, GY;Kim, J;Bae, JH;Park, YS; Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer Scientific reports 2023-05-11 [PMID: 37169953] (Flow Cytometry, Human) Flow Cytometry Human
Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human) Flow Cytometry Human
Jung HY, Lee DK, Lee M et al. ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer Oncoimmunology 2023-03-17 [PMID: 36950218]
NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects Nature, 2023-01-11;613(7945):743-750. 2023-01-11 [PMID: 36631610] (Neutralization, Human) Neutralization Human
Amanda Cooper, Adam Sidaway, Abishek Chandrashekar, Elizabeth Latta, Krishnendu Chakraborty, Jingyou Yu et al. A genetically engineered, stem-cell-derived cellular vaccine Cell Reports Medicine 2022-12-20 [PMID: 36480934]
S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 2022-02-25;12(1):3234. 2022-02-25 [PMID: 35217717] (Neutralization, Human) Neutralization Human
N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 2022-01-25;12(1):1341. 2022-01-25 [PMID: 35079096] (Flow Cytometry, Human) Flow Cytometry Human
S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021-09-04;12(9):836. 2021-09-04 [PMID: 34482362] (Flow Cytometry, Human) Flow Cytometry Human
E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021-05-03;17(5):e1008807. 2021-05-03 [PMID: 33939764] (Flow Cytometry, Human) Flow Cytometry Human
Show All 65 Publications.

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Bioinformatics

Gene Symbol ULBP1