![Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-278202415242628.jpg "Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MDA-MB-468 cell lysate (20 ug/Lane) Lane 4: SH-SY5Y cell lysate (20 ug/Lane) Lane 5: HUVEC cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane) Lane 9: COS-1 cell lysate (20 ug/Lane) Lane 10: Neuro-2a cell lysate (20 ug/Lane) Lane 11: NIH/3T3 cell lysate (20 ug/Lane) Lane 12: C6 cell lysate (20 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | PSH02-52 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human NF2/Merlin aa 1-350 / 595 (P35240). (Uniprot: P35240) |
| Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NF2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | NF2 |
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Immunocytochemistry analysis of HUVEC cells labeling NF2/Merlin with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-2920241192366.jpg "Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Immunocytochemistry analysis of HUVEC cells labeling NF2/Merlin with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Flow cytometric analysis of NIH/3T3 cells labeling NF2/Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32590_rabbit-nf2-merlin-mab-psh02-52-29202416372197.jpg "Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Flow cytometric analysis of NIH/3T3 cells labeling NF2/Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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