![Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0006.jpg "Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.")
| Reactivity | Rt, ChSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Purified rat Neurofilament Heavy construct containing most of the tandem KSP repeats [UniProt# P16884] |
| Marker | Neuronal Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NEFH |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This 200kDa Neurofilament Heavy antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 200 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antisera |
| Preservative | No Preservative |
| Purity | Unpurified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-H Antibody (NBP1-05215)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NBP1-05215] - Shows mixed neuron/glial rat brain cultures stained with NBP1-05215 (green) and chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) NBP1-05198 (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05215-img0004.jpg "Immunocytochemistry/Immunofluorescence: NF-H Antibody [NBP1-05215] - Shows mixed neuron/glial rat brain cultures stained with NBP1-05215 (green) and chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) NBP1-05198 (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.")
![Immunohistochemistry Free-Floating: NF-H Antibody [NBP1-05215] - Analysis of a mouse hippocampus section stained with NF-H antibody, dilution 1:2,000 (Red), and costained with mouse myelin basic protein mAb (MBP), dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Free-Floating-NBP1-05215-img0007.jpg "Immunohistochemistry Free-Floating: NF-H Antibody [NBP1-05215] - Analysis of a mouse hippocampus section stained with NF-H antibody, dilution 1:2,000 (Red), and costained with mouse myelin basic protein mAb (MBP), dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.")
![Western Blot: NF-H Antibody [NBP1-05215] - Blot of rat spinal cord extract blotted with NBP1-05215. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated dendritic and perilaryal form of this protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0005.jpg "Western Blot: NF-H Antibody [NBP1-05215] - Blot of rat spinal cord extract blotted with NBP1-05215. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated dendritic and perilaryal form of this protein.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")