Nephrin Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 8 applications: Flow Cytometry, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Western Blot.

Showing Publications 1 - 10 of 51. Show All 51 Publications. Collapse Publications.

Publications using AF4269	Applications	Species
Shi, M;Crouse, B;Sundaram, N;Shakked, NP;Ester, L;Zhang, W;Janakiram, V;Kopan, R;Helmrath, MA;Bonventre, JV;McCracken, KW; Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud bioRxiv : the preprint server for biology 2024-09-20 [PMID: 39345524] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Xi, G;Lamba, SA;Mysh, M;Poulton, JS; Oxidative Stress Contributes to Slit Diaphragm Defects Caused by Disruption of Endocytosis Kidney international reports 2024-02-01 [PMID: 38344712] (Western Blot, Human)	Western Blot	Human
Butt, L;Unnersjö-Jess, D;Reilly, D;Hahnfeldt, R;Rinschen, MM;Bozek, K;Schermer, B;Benzing, T;Höhne, M; In vivo characterization of a podocyte-expressed short podocin isoform BMC nephrology 2023-12-19 [PMID: 38114895] (IHC, Transgenic Mouse)	IHC	Transgenic Mouse
Maria Elena Melica, Maria Lucia Angelotti, Giulia Antonelli, Anna J. Peired, Carolina Conte, Letizia De Chiara et al. Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes BIO-PROTOCOL 2023-08-20 [PMID: 37638296]
Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM; An integrated organoid omics map extends modeling potential of kidney disease Nature communications 2023-08-14 [PMID: 37580326] (ICC/IF, Human)	ICC/IF	Human
Charrin E, Dabaghie D, Sen I et al. Soluble Klotho protects against glomerular injury through regulation of ER stress response Communications biology 2023-02-22 [PMID: 36813870]
Lonneke H. Gaykema, Rianne Y. van Nieuwland, Mette C. Dekkers, Mieke F. van Essen, Sebastiaan Heidt, Arnaud Zaldumbide et al. Inhibition of complement activation by CD55 overexpression in human induced pluripotent stem cell derived kidney organoids Frontiers in Immunology 2023-01-12 [PMID: 36713440]
David H. Vandorpe, John F. Heneghan, Joshua S. Waitzman, Gizelle M. McCarthy, Angelo Blasio, Jose M. Magraner et al. Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes Pflügers Archiv - European Journal of Physiology 2023-03-01 [PMID: 36449077]
G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation Cell Stem Cell, 2022-11-03;29(11):1580-1593.e7. 2022-11-03 [PMID: 36332571] (IHC, Human)	IHC	Human
Keonhyeok Park, Jong Young Lee, Soo Young Lee, Iljoo Jeong, Seo-Yeon Park, Jin Won Kim et al. Deep learning predicts the differentiation of kidney organoids derived from human induced pluripotent stem cells Kidney Research and Clinical Practice 2023-01-31 [PMID: 36328994]
Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids Nature Communications 2022-10-08 [PMID: 36209212]
Gangqi Wang, Bram Heijs, Sarantos Kostidis, Ahmed Mahfouz, Rosalie G. J. Rietjens, Roel Bijkerk et al. Analyzing cell-type-specific dynamics of metabolism in kidney repair Nature Metabolism 2022-08-25 [PMID: 36008550]
Hyunjae Chung, Maxwell P. Bui-Marinos, Waleed Rahmani, Jennifer A. Corcoran, Justin Chun Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2 STAR Protocols 2022-09-01 [PMID: 35990736]
Julie Bejoy, Justin M. Farry, Jennifer L. Peek, Mariana C. Cabatu, Felisha M. Williams, Richard C. Welch et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease Stem Cell Research & Therapy 2022-12-01 [PMID: 35883199]
AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel Transplanted organoids empower human preclinical assessment of drug candidate for the clinic Science Advances, 2022-07-06;8(27):eabj5633. 2022-07-06 [PMID: 35857479] (Flow Cytometry, Human)	Flow Cytometry	Human
N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days Cells, 2022-06-02;11(11):. 2022-06-02 [PMID: 35681517] (IHC, Mouse)	IHC	Mouse
Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids bioRxiv 2023-04-15 [PMID: 35665006]
Garreta E, Prado P, Stanifer ML et al. A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells Cell Metabolism 2022-06-07 [PMID: 35561674]
Jitske Jansen, Bartholomeus T. van den Berge, Martijn van den Broek, Rutger J. Maas, Deniz Daviran, Brigith Willemsen et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling Development 2022-05-01 [PMID: 35417019]
Lawrence M, Elhendawi M, Morlock M et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity iScience 3/1/2022 [PMID: 35243244]
Waleed Rahmani, Hyunjae Chung, Sarthak Sinha, Maxwell P. Bui-Marinos, Rohit Arora, Arzina Jaffer et al. Attenuation of SARS-CoV-2 infection by losartan in human kidney organoids iScience 2022-02-18 [PMID: 35106453]
J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids Cell Stem Cell, 2021-12-25;29(2):217-231.e8. 2021-12-25 [PMID: 35032430] (IHC, Human)	IHC	Human
L Miesen, P Bándi, B Willemsen, F Mooren, T Strieder, E Boldrini, V Drenic, J Eymael, R Wetzels, J Lotz, N Weiss, E Steenberge, TH van Kuppev, M van Erp, J van der La, N Endlich, MJ Moeller, JFM Wetzels, J Jansen, B Smeets Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis Disease Models & Mechanisms, 2022-03-14;0(0):. 2022-03-14 [PMID: 34927672] (IHC, Rat)	IHC	Rat
R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis Cell Stem Cell, 2021-11-29;0(0):. 2021-11-29 [PMID: 34847364] (IHC, Human)	IHC	Human
KS Lee, J Lee, P Lee, BC Jeon, MY Song, S Kwak, J Lee, JS Kim, DJ Kim, JH Kim, VL Tesh, MS Lee, SK Park Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host Embo Molecular Medicine, 2021-11-29;0(0):e14678. 2021-11-29 [PMID: 34842355] (IHC, Human)	IHC	Human
Julie Bejoy, Eddie Spencer Qian, Lauren Elizabeth Woodard Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells STAR Protocols 2021-12-01 [PMID: 34746862]
S Ueda, T Tominaga, A Ochi, A Sakurai, K Nishimura, E Shibata, S Wakino, M Tamaki, K Nagai TGF-beta1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction Scientific Reports, 2021-11-04;11(1):21643. 2021-11-04 [PMID: 34737348] (IHC, Human)	IHC	Human
JW Kim, HW Kim, SA Nam, JY Lee, HJ Cho, TM Kim, YK Kim Human kidney organoids reveal the role of glutathione in Fabry disease Experimental & Molecular Medicine, 2021-10-15;0(0):. 2021-10-15 [PMID: 34654880] (IHC, Human)	IHC	Human
N Mangold, J Pippin, D Unnersjoe-, S Koehler, S Shankland, S Brähler, B Schermer, T Benzing, PT Brinkkoett, H Hagmann The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development Cells, 2021-09-18;10(9):. 2021-09-18 [PMID: 34572114] (IHC, Mouse)	IHC	Mouse
Sun-Sang J Sung Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli BIO-PROTOCOL 2020-07-05 [PMID: 33659333]
Qizheng Wang, Yucui Xiong, Sheng Zhang, Yufei Sui, Cunlai Yu, Peng Liu et al. The Dynamics of Metabolic Characterization in iPSC-Derived Kidney Organoid Differentiation via a Comparative Omics Approach Frontiers in Genetics 2/10/2021 [PMID: 33643392]
Sander V, Przepiorski A, Crunk AE et al. Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells STAR protocols 12/18/2020 [PMID: 33377044]
K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020-12-15;33(11):108514. 2020-12-15 [PMID: 33326782] (IHC, Human)	IHC	Human
Jin Won Kim, Sun Ah Nam, Eunjeong Seo, Jong Young Lee, Dohui Kim, Ji Hyeon Ju et al. Human kidney organoids model the tacrolimus nephrotoxicity and elucidate the role of autophagy The Korean Journal of Internal Medicine 2021-11-01 [PMID: 32972120]
J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata Transcription factor 21 expression in injured podocytes of glomerular diseases Sci Rep, 2020-07-13;10(1):11516. 2020-07-13 [PMID: 32661376] (IHC, Human)	IHC	Human
Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2. Cell, 2020-04-24;181(4):905-913.e7. 2020-04-24 [PMID: 32333836] (IHC, Human)	IHC	Human
A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation Nat Commun, 2019-11-29;10(1):5462. 2019-11-29 [PMID: 31784515] (ICC, Mouse)	ICC	Mouse
Vanslambrouck J M, Wilson S B et al. A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids. J Am Soc Nephrol 1/10/2019 [PMID: 31492807]
Sara E Howden, Jessica M Vanslambrouck, Sean B Wilson, Ker Sin Tan, Melissa H Little Reporter‐based fate mapping in human kidney organoids confirms nephron lineage relationships and reveals synchronous nephron formation EMBO reports 2019-04-01 [PMID: 30858339]
Santhosh V. Kumar, Pei X. Er, Kynan T. Lawlor, Ali Motazedian, Michelle Scurr, Irene Ghobrial et al. Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells Development 2019-03-01 [PMID: 30846463]
Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]
JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler Organoid single cell profiling identifies a transcriptional signature of glomerular disease JCI Insight, 2019-01-10;4(1):. 2019-01-10 [PMID: 30626756] (IHC-P, Human)	IHC-P	Human
B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little Evaluation of variability in human kidney organoids Nat. Methods, 2018-12-20;16(1):79-87. 2018-12-20 [PMID: 30573816] (IHC, Mouse)	IHC	Mouse
LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening Nat Commun, 2018-12-04;9(1):5167. 2018-12-04 [PMID: 30514835] (Human)		Human
H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics Cell Stem Cell, 2018-11-15;0(0):. 2018-11-15 [PMID: 30449713] (IHC-Fr, Human)	IHC-Fr	Human
Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies. Drug Metab Dispos, 2018-08-31;46(11):1703-1711. 2018-08-31 [PMID: 30171163] (ICC, Human)	ICC	Human
Stefan M. Czerniecki, Nelly M. Cruz, Jennifer L. Harder, Rajasree Menon, James Annis, Edgar A. Otto et al. High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping Cell Stem Cell 2018-06-01 [PMID: 29779890]
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions Sci Rep, 2016-11-16;6(0):37172. 2016-11-16 [PMID: 27849017] (IHC-P, Human)	IHC-P	Human
Minoru Takasato, Pei X Er, Han S Chiu, Melissa H Little Generation of kidney organoids from human pluripotent stem cells Nature Protocols 2016-09-01 [PMID: 27560173]
Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature, 2015-10-07;526(7574):564-8. 2015-10-07 [PMID: 26444236] (IHC, Human)	IHC	Human
Joseph P. Gaut, Masato Hoshi, Sanjay Jain, Helen Liapis Claudin 1 and nephrin label cellular crescents in diabetic glomerulosclerosis Human Pathology 2014-03-01 [PMID: 24529330]
Show All 51 Publications. Collapse Publications.

Array AF4269

• Nephrin Antibodies
• Nephrin ELISA Kits
• Nephrin Lysate
• Nephrin Protein
• Nephrin ELISA
• Nephrin Proteins and Enzymes