![Knockout Validated: NDUFV1 Antibody [NBP1-33074] - Wild-type (WT) and NDUFV1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Western-Blot-NBP1-33074-img0006.jpg "Knockout Validated: NDUFV1 Antibody [NBP1-33074] - Wild-type (WT) and NDUFV1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, Ze, Rt, Bv, Ch, Pm, XpSpecies Glossary |
| Applications | WB, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFV1. The exact sequence is proprietary. |
| Localization | Mitochondrion inner membrane, Peripheral membrane protein, Matrix side |
| Predicted Species | Rat (97%), Chicken (95%), Xenopus (90%), Bovine (98%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NDUFV1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/16250.jpg)
![Immunocytochemistry beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/beta-III_Tubulin_MAB1195_Immunocytochemistry_6556.jpg)
![Immunohistochemistry beta-III Tubulin Antibody (TuJ-1) [Unconjugated] - Neuron-Specific](https://images.novusbio.com/images2/betaIII_Tubulin_MAB1195_Immunohistochemistry_22757.jpg)
| Publication using NBP1-33074 | Applications | Species |
|---|---|---|
| Wu, Z, Tantray, I Et al. MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure. Mol Cell 2019-08-22 [PMID: 31378462] (ICC/IF, Mouse) | ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NDUFV1 |
![Western Blot: NDUFV1 Antibody [NBP1-33074] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Western-Blot-NBP1-33074-img0013.jpg "Western Blot: NDUFV1 Antibody [NBP1-33074] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Analysis of paraffin-embedded zebrafish tissue, using NDUFV1 antibody [N3C3] at 1:300 dilution.](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Immunohistochemistry-Paraffin-NBP1-33074-img0009.jpg "Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Analysis of paraffin-embedded zebrafish tissue, using NDUFV1 antibody [N3C3] at 1:300 dilution.")
![Western Blot: NDUFV1 Antibody [NBP1-33074] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Western-Blot-NBP1-33074-img0011.jpg "Western Blot: NDUFV1 Antibody [NBP1-33074] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - U373 xenograft, using NDUFV1 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Immunohistochemistry-Paraffin-NBP1-33074-img0002.jpg "Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - U373 xenograft, using NDUFV1 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Immunohistochemistry-Paraffin-NBP1-33074-img0007.jpg "Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NDUFV1-Antibody-Immunohistochemistry-Paraffin-NBP1-33074-img0008.jpg "Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")