![Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 expression in transfected 293T cell line by NDUFS3 monoclonal antibody (M02), clone 1D6.Lane 1: NDUFS3 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-1D6-Western-Blot-H00004722-M02-img0008.jpg "Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 expression in transfected 293T cell line by NDUFS3 monoclonal antibody (M02), clone 1D6.Lane 1: NDUFS3 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KD |
| Clone | 1D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | NDUFS3 (AAH00617, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAAVARLWWRGILGASALTRGTGRPSVLLLPVRRESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK |
| Specificity | NDUFS3 - NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NDUFS3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, ELISA and RNAi Validation. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of monoclonal antibody to NDUFS3 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-1D6-Immunohistochemistry-Paraffin-H00004722-M02-img0005.jpg "Immunohistochemistry-Paraffin: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of monoclonal antibody to NDUFS3 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.")
![Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 over-expressed 293 cell line, cotransfected with NDUFS3 Validated Chimera RNAi ( Cat # H00004722-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS3 monoclonal antibody (M02), clone 1D6 (Cat # H00004722-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-1D6-Western-Blot-H00004722-M02-img0007.jpg "Western Blot: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 over-expressed 293 cell line, cotransfected with NDUFS3 Validated Chimera RNAi ( Cat # H00004722-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS3 monoclonal antibody (M02), clone 1D6 (Cat # H00004722-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunoprecipitation: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 transfected lysate using anti-NDUFS3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NDUFS3 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NDUFS3-Antibody-1D6-Immunoprecipitation-H00004722-M02-img0006.jpg "Immunoprecipitation: NDUFS3 Antibody (1D6) [H00004722-M02] - Analysis of NDUFS3 transfected lysate using anti-NDUFS3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NDUFS3 MaxPab rabbit polyclonal antibody.")
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