![Knockout Validated: NDUFS2 Antibody (OTI1D5) [NBP2-46127] - Lysates of HAP1 (WT and NDUFS2 KO) were prepared and 40 ug of protein were processed for immunoblot with NDUFS2 antibody (OTI1D5). The Ponceau stained transfers of the blot are shown. Antibody dilution used: NBP2-46127 at 1/2000. Predicted band size: 52 kDa. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/NDUFS2-Antibody-OTI1D5-Knockout-Validated-NBP2-46127-img0013.jpg "Knockout Validated: NDUFS2 Antibody (OTI1D5) [NBP2-46127] - Lysates of HAP1 (WT and NDUFS2 KO) were prepared and 40 ug of protein were processed for immunoblot with NDUFS2 antibody (OTI1D5). The Ponceau stained transfers of the blot are shown. Antibody dilution used: NBP2-46127 at 1/2000. Predicted band size: 52 kDa. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, KO |
| Clone | OTI1D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 205-463 of human NDUFS2 (NP_004541) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NDUFS2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 49.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-46127 | Applications | Species |
|---|---|---|
| Pang Y, Lu Y, et al. Targeting NAD+/PARP DNA Repair Pathway as a Novel Therapeutic Approach to SDHB-Mutated Cluster I Pheochromocytoma and Paraganglioma. Clin Cancer Res 2018-07-15 [PMID: 29636359] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for NDUFS2 Antibody (NBP2-46127)Find related products by research area.
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| Gene Symbol | NDUFS2 |
![Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Western-Blot-NBP2-46127-img0011.jpg "Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2.")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0010.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of A549 cell lysate (35ug) by using NDUFS2 monoclonal antibody.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Western-Blot-NBP2-46127-img0012.jpg "Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of A549 cell lysate (35ug) by using NDUFS2 monoclonal antibody.")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human colon tissue using. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0001.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human colon tissue using. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0002.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0003.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0004.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0005.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0006.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0007.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0008.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0009.jpg "Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![N/A Endothelin-1 [HRP]](https://images.novusbio.com/images2/Endothelin-1_QET00_ELISA_432.jpg)
![N/A Endothelin-1 [HRP]](https://images.novusbio.com/images2/DATA_Endothelin1_QET00_ELISA_1074.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")