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NCKIPSD Antibody - BSA Free

Images

 
Western Blot: NCKIPSD Antibody [NBP2-56886] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: NCKIPSD Antibody [NBP2-56886] - Staining of human cell line HeLa shows localization to plasma membrane & cytosol.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

NCKIPSD Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPLPSSQIPPQP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCKIPSD
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NCKIPSD Recombinant Protein Antigen (NBP2-56886PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NCKIPSD Antibody - BSA Free

  • 54 kDa vimentin-interacting protein
  • 90 kDa SH3 protein interacting with Nck
  • AF3p21
  • AF3P21DIP-1
  • dia interacting protein
  • Dia-interacting protein 1
  • diaphanous protein interacting protein
  • Diaphanous protein-interacting protein
  • DIP
  • DIP1
  • MGC23891
  • NCK interacting protein with SH3 domain
  • ORF1
  • SPIN90VIP54
  • WASLBP
  • WASP-interacting SH3-domain protein
  • WISHSH3 adapter protein SPIN90
  • Wisk90 kDa

Background

NCKIPSD is encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NCKIPSD