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NCKIPSD Antibody

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Western Blot: NCKIPSD Antibody [NBP1-42664] - Detection of Human SPIN90 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody NBP1-42664 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: NCKIPSD Antibody [NBP1-42664] - Detection of Human SPIN90 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry: NCKIPSD Antibody [NBP1-42664] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SPIN90 used at a dilution of 1:500 (0.4ug/ml). Detection: DAB. Counterstain: ...read more
NCKIPSD Antibody [NBP1-42664] - Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

      

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NCKIPSD Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 672 and 722 of human SH3 protein interacting with Nck, 90 kDa using the numbering given in entry NP_057537.1 (GeneID 51517).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCKIPSD
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCKIPSD Antibody

  • 54 kDa vimentin-interacting protein
  • 90 kDa SH3 protein interacting with Nck
  • AF3p21
  • AF3P21DIP-1
  • dia interacting protein
  • Dia-interacting protein 1
  • diaphanous protein interacting protein
  • Diaphanous protein-interacting protein
  • DIP
  • DIP1
  • MGC23891
  • NCK interacting protein with SH3 domain
  • ORF1
  • SPIN90VIP54
  • WASLBP
  • WASP-interacting SH3-domain protein
  • WISHSH3 adapter protein SPIN90
  • Wisk90 kDa

Background

The protein encoded by this gene is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NCKIPSD
Entrez