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NCF1 Antibody

Images

 
Western Blot: NCF1 Antibody [NBP2-19487] - Sample (50 ug of whole cell lysate) A: mouse spleen, 10% SDS PAGE gel, diluted at 1:500.
Western Blot: NCF1 Antibody [NBP2-19487] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:5000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NCF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCF1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (84%), Rabbit (86%), Rat (82%), Bovine (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for NCF1 Antibody

  • 47 kDa neutrophil oxidase factor
  • FLJ79451
  • NADPH oxidase organizer 2
  • NCF-1
  • NCF1A
  • neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein
  • neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1)
  • neutrophil cytosolic factor 1
  • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
  • Neutrophil NADPH oxidase factor 1
  • Nox organizer 2
  • NOXO2NCF-47K
  • Nox-organizing protein 2
  • p47phox
  • SH3 and PX domain-containing protein 1A
  • SH3PXD1Ap47-phox

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on NCF1.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by ...  Read full blog post.

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Bioinformatics

Gene Symbol NCF1
Uniprot