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NCAPD3 Antibody

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Western Blot: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunoprecipitation: NCAPD3 Antibody [NB100-1573] - Detection of human CAP-D3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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NCAPD3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1450 and the C-terminus (residue 1507) of human Chromosome-Associated Protein D3 using the numbering given in entry NP_056076.1 (GeneID 23310).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCAPD3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30455981).
Publications
Read Publications using
NB100-1573 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCAPD3 Antibody

  • CAPD3
  • CAP-D3
  • FLJ42888
  • hCAP-D3condensin-2 complex subunit D3
  • hcp-6
  • hHCP-6
  • KIAA0056MGC104671
  • Non-SMC condensin II complex subunit D3
  • non-SMC condensin II complex, subunit D3

Background

CAP-D3 is a subunit of the heteropentameric condensin II complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II, and aids in the proper segregation of sister chromatids. In the condensin II complex CAP-D3 is regulated by protein phosphatase 2A (PP2A) and may participate in centromere resolution. Alternate designations of CAP-D3 include condensin-II complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3, CAPD3, hcp-6, hHCP-6, FLJ42888, MGC1046871, and KIAA0056.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCAPD3 Antibody (NB100-1573)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NCAPD3
Entrez
Uniprot