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NAP1L4 Antibody

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Western Blot: NAP1L4 Antibody [NBP2-36543] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NAP-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: NAP1L4 Antibody [NBP2-36543] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: ...read more
Independent Antibodies: Immunoprecipitation: NAP1L4 Antibody [NBP2-36543] - Detection of human NAP-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-36543AF350
Alexa Fluor 405
NBP2-36543AF405
Alexa Fluor 488
NBP2-36543AF488
Alexa Fluor 532
NBP2-36543AF532
Alexa Fluor 594
NBP2-36543AF594
Alexa Fluor 647
NBP2-36543AF647
Alexa Fluor 700
NBP2-36543AF700
Alexa Fluor 750
NBP2-36543AF750
Biotin
NBP2-36543B
DyLight 350
NBP2-36543UV
DyLight 405
NBP2-36543V
DyLight 488
NBP2-36543G
DyLight 550
NBP2-36543R
DyLight 594
NBP2-36543DL594
DyLight 650
NBP2-36543C
DyLight 680
NBP2-36543FR
DyLight 755
NBP2-36543IR
HRP
NBP2-36543H
Janelia Fluor 549
NBP2-36543JF549
Janelia Fluor 646
NBP2-36543JF646
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NAP1L4 Antibody Summary

Immunogen
The immunogen recognized by the antibody maps to a region between residue 325 to 375 of human Nucleosome Assembly Protein 2 using the numbering given in entry NP_005960.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAP1L4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NAP1L4 Antibody

  • hNAP2
  • MGC4565
  • NAP-2
  • NAP2L
  • NAP2NAP1L4b
  • nucleosome assembly protein 1-like 4
  • nucleosome assembly protein 1-like 4b
  • Nucleosome assembly protein 2

Background

NAP1L4 (Nucleosome Assembly Protein 1-Like 4) is thought to have involvement as a histone chaperone. Due to its location near the imprinted gene domain of 11p15.5, NAP1L4 has been studied in relation to Wilms tumor, Beckwith-Wiedemann syndrome, rhabdomyosarcoma and several forms of cancer including breast, lung and ovarian cancer. NAP1L4 is known to have interactions with HIST1H4A, HIST2H4A, HIST1H4AB, HIST1H4C and HIST1H4D.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NAP1L4
Uniprot