![Western Blot: MuSK Antibody [NB120-5619] - Analysis of 3ng of muscle-specific kinase antigen and 10ul PageRuler Plus Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/MuSK-Antibody-Western-Blot-NB120-5619-img0012.jpg "Western Blot: MuSK Antibody [NB120-5619] - Analysis of 3ng of muscle-specific kinase antigen and 10ul PageRuler Plus Prestained Protein Ladder per well.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant protein containing residues 210-304 from the extracellular domain of rat MuSK |
| Localization | Membrane; Single-pass type I membrane protein |
| Specificity | Detects MUSK from mouse and rat tissue samples as well as COS7 cells transfected with rat MUSK. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MUSK |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA and 30% glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MuSK Antibody (NB120-5619)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/MuSK-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5619-img0009.jpg "Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on mouse skeletal muscle tissue.](http://images.novusbio.com/fullsize/MuSK-Antibody-Immunohistochemistry-Paraffin-NB120-5619-img0011.jpg "Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on mouse skeletal muscle tissue.")
![Western Blot: MuSK Antibody [NB120-5619] - Analysis of Rat MuSK transfected COS7 cell lysate.](http://images.novusbio.com/fullsize/MuSK-Antibody-Western-Blot-NB120-5619-img0007.jpg "Western Blot: MuSK Antibody [NB120-5619] - Analysis of Rat MuSK transfected COS7 cell lysate.")
![Western Blot: MuSK Antibody [NB120-5619] - Analysis of 30ug of rat skeletal muscle tissue lysate and 10ul PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.](http://images.novusbio.com/fullsize/MuSK-Antibody-Western-Blot-NB120-5619-img0013.jpg "Western Blot: MuSK Antibody [NB120-5619] - Analysis of 30ug of rat skeletal muscle tissue lysate and 10ul PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in L6 rat myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/MuSK-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5619-img0008.jpg "Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in L6 rat myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on rat skeletal muscle tissue.](http://images.novusbio.com/fullsize/MuSK-Antibody-Immunohistochemistry-Paraffin-NB120-5619-img0010.jpg "Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on rat skeletal muscle tissue.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")