![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0014.jpg "Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human mtTFA Recombinant protein. |
| Localization | Mitochondrion |
| Predicted Species | Chimpanzee (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TFAM |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for mtTFA Antibody (NBP2-19438)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0012.jpg "Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg "Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody ) diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0003.jpg "Western Blot: mtTFA Antibody [NBP2-19438] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody ) diluted at 1:1000.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg "Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0011.jpg "Western Blot: mtTFA Antibody [NBP2-19438] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0004.jpg "Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0006.jpg "Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.")
![Immunoprecipitation: mtTFA Antibody [NBP2-19438] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19438-img0008.jpg "Immunoprecipitation: mtTFA Antibody [NBP2-19438] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")