mtTFA Antibodies
We offer mtTFA Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each mtTFA Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our mtTFA Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the mtTFA Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our mtTFA polyclonal antibodies and browse our mtTFA monoclonal antibody catalog.
Alternate Names for mtTFA Antibodies
anti-mtTFA antibody, anti-TFAM antibody, anti-mitochondrial transcription factor A antibody, anti-MTTF1 antibody, anti-TCF-6 antibody, anti-TCF6L1 antibody, anti-TCF6L2Mitochondrial transcription factor 1 antibody, anti-TCF6TCF6L3 antibody, anti-Transcription factor 6 antibody, anti-Transcription factor 6-like 2 (mitochondrial transcription factor) antibody, anti-Transcription factor 6-like 2 antibody, anti-transcription factor A, mitochondrial antibody
9 Results for "mttfa" in Primary Antibodies
![Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Simple-Western-NBP1-71648-img0008.jpg "Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide (EtBr) for 8A weeks. Transfection with 30A nmol/L negative or TFAM siRNA for 8A days. TFAM protein relative toI2-actin. D, MtDNA levels. E, LPS-induced TNF-I+-and IL-8 release. Image collected and cropped by Citeab from the following publication (Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-g. J Allergy Clin Immunol (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for 0AE](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0013.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunohistochemistry-Paraffin-NBP1-71648-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0003.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Total protein from Hela and U937 were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-mtTFA NBP1-71648 in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0017.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0009.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19437-img0008.jpg "Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human colon cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0007.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19437-img0001.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human esophagus cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0006.jpg)
![Immunoprecipitation: mtTFA Antibody [NBP2-19437] - A. 293T whole cell lysate/extract B. 40 ug 293T whole cell lysate/extract. C.. Control with 2 ug of preimmune rabbit IgG.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19437-img0004.jpg)
![HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA stained by mtTFA antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunofluorescence-22320239914.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0014.jpg "Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0012.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody ) diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0003.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0006.jpg)
![Immunoprecipitation: mtTFA Antibody [NBP2-19438] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19438-img0008.jpg)
![Western Blot: mtTFA Antibody [NBP1-86959] - Analysis using Anti-TFAM antibody NBP1-86959 (A) shows similar pattern to independent antibody NBP2-56077 (B).](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP1-86959-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: mtTFA Antibody [NBP1-86959] - Analysis using Anti-TFAM antibody NBP1-86959 (A) shows similar pattern to independent antibody NBP2-56077 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP1-86959] - Staining of human cell line U-2 OS shows positivity in mitochondria.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86959-img0014.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human testis shows strong granular cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP1-86959] - Analysis in human cell lines Caco-2 and HeLa using anti-TFAM antibody. Corresponding TFAM RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP1-86959-img0015.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human breast shows strong cytoplasmic positivity in granular pattern in glandular cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0007.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0010.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0011.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0012.jpg)
![Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Western-Blot-NBP3-16154-img0003.jpg "Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunofluorescence analysis of U-2 OS cells using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Immunocytochemistry-Immunofluorescence-NBP3-16154-img0002.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunohistochemistry of paraffin-embedded human colon using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Immunohistochemistry-Paraffin-NBP3-16154-img0001.jpg)
![Chromatin Immunoprecipitation: mtTFA Antibody (8O1U0) [NBP3-16154] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using mtTFA antibody (NBP3-16154) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Chromatin-Immunoprecipitation-NBP3-16154-img0004.jpg)
![Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Western-Blot-NBP3-19504-img0003.jpg "Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunofluorescence of mtTFA (green) in Hela cells using NBP3-19504 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Immunocytochemistry-Immunofluorescence-NBP3-19504-img0001.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using NBP3-19504 at dilution 1/1](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Immunohistochemistry-Paraffin-NBP3-19504-img0002.jpg)
![Western Blot: mtTFA Antibody [NBP2-56077] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-56077-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-56077] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: mtTFA Antibody [NBP2-56077] - Analysis using Anti-TFAM antibody NBP2-56077 (A) shows similar pattern to independent antibody NBP1-86959 (B).](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-56077-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-56077] - Staining of human cell line RH-30 shows localization to mitochondria.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56077-img0001.jpg)