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TFB2M Antibody

Images

 
Western Blot: TFB2M Antibody [NBP1-78283] - (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TFB2M Antibody [NBP1-78283] - 5ug/ml staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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TFB2M Antibody Summary

Immunogen
Peptide with sequence KHCFGRRSATVIDH corresponding to internal region according to NP_071761.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TFB2M
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.1-0.3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 ug/ml
Application Notes
Western blot: Approx 45kDa band observed in Human Lung lysates (calculated MW of 45.3kDa according to NP_071761.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Colon.
Publications
Read Publication using NBP1-78283.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TFB2M Antibody

  • dimethyladenosine transferase 2, mitochondrial
  • EC 2.1.1.-
  • FLJ22661
  • FLJ23182
  • HCV NS5A-transactivated protein 5
  • Hepatitis C virus NS5A-transactivated protein 5
  • Hkp1
  • h-mtTFB
  • h-mtTFB2
  • hTFB2M
  • Mitochondrial 12S rRNA dimethylase 2
  • Mitochondrial transcription factor B2
  • mtTFB2
  • NS5ATP5
  • S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
  • transcription factor B2, mitochondrial

Background

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TFB2M Antibody (NBP1-78283)(1)

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Bioinformatics

Gene Symbol TFB2M