![Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-western-blot-283202415553..jpg "Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence RGDLNDCFIPCTPK corresponding to internal region according to NP_005947.2. |
| Epitope | RGDLNDCFIPCTPK |
| Marker | Cytoplasm Marker |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | MTHFD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Approx. 100kDa band observed in lysates of cell lines Jurkat and MOLT4 and approx.110kDa in lysates of cell line MCF7 (calculated MW of 102kDa according to NP_005947.2). |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for MTHFD1 Antibody (NBP1-06054)Find related products by research area.
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| Gene Symbol | MTHFD1 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-immunohistochemistry-283202415110..jpg "Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-immunocytochemistry-immunofluorescence-2832024151738..jpg "Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
