| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82611-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46781-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99164-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97940-50ul | |||
![Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0012.jpg)
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0017.jpg)
![Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0009.jpg)
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0014.jpg)
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0015.jpg)
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0016.jpg)
![Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-western-blot-283202415553..jpg "Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-immunohistochemistry-283202415110..jpg)
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp1-06054_goat-polyclonal-mthfd1-antibody-immunocytochemistry-immunofluorescence-2832024151738..jpg)
![Western Blot: MTHFD1 Antibody [NBP1-82611] - Analysis using Anti-MTHFD1 antibody NBP1-82611 (A) shows similar pattern to independent antibody NBP1-82612 (B).](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82611-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MTHFD1 Antibody [NBP1-82611] - Analysis using Anti-MTHFD1 antibody NBP1-82611 (A) shows similar pattern to independent antibody NBP1-82612 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82611] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82611-img0007.jpg)
![Western Blot: MTHFD1 Antibody [NBP1-82610] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82610-img0003.jpg "Western Blot: MTHFD1 Antibody [NBP1-82610] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82610] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82610-img0001.jpg)
![Western Blot: MTHFD1 Antibody [NBP1-82610] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Western-Blot-NBP1-82610-img0005.jpg)
![Western Blot: MTHFD1 Antibody [NBP3-38574] - Western blot analysis of various lysates using MTHFD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38574_rabbit-mthfd1-pab-912202414154366.jpg "Western Blot: MTHFD1 Antibody [NBP3-38574] - Western blot analysis of various lysates using MTHFD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunocytochemistry/ Immunofluorescence: MTHFD1 Antibody [NBP3-38574] - Immunofluorescence analysis of U-2 OS cells using MTHFD1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38574_rabbit-mthfd1-pab-912202415152810.jpg)
![Western Blot: MTHFD1 Antibody [NBP2-46781] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.](http://images.novusbio.com/fullsize/MTHFD1L-Antibody-Western-Blot-NBP2-46781-img0002.jpg "Western Blot: MTHFD1 Antibody [NBP2-46781] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.")
![Immunohistochemistry: MTHFD1 Antibody [NBP2-46781] - Staining of human Analysis of human liver tissue. shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/MTHFD1L-Antibody-Immunohistochemistry-NBP2-46781-img0001.jpg)
![Immunoprecipitation:MTHFD1 AntibodyNBP3-30621] -Detection of human MTHFD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFD1 antibody NBP3-30621 (lot NBP3-30621-1) used for IP at 6 µg per reaction. MTHFD1 was also immunoprecipitated by rabbit anti-MTHFD1 antibodies. For blotting immunoprecipitated MTHFD1, NBP3-30621 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30621_rabbit-mthfd1-pab-301220241556515.jpg "Immunoprecipitation:MTHFD1 AntibodyNBP3-30621] -Detection of human MTHFD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFD1 antibody NBP3-30621 (lot NBP3-30621-1) used for IP at 6 µg per reaction. MTHFD1 was also immunoprecipitated by rabbit anti-MTHFD1 antibodies. For blotting immunoprecipitated MTHFD1, NBP3-30621 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:MTHFD1 AntibodyNBP3-29959] -Detection of human MTHFD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFD1 antibody NBP3-29959 (lot NBP3-29959-1) used for IP at 6 µg per reaction. MTHFD1 was also immunoprecipitated by rabbit anti-MTHFD1 antibodies for blotting immunoprecipitated MTHFD1, NBP3-29959 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29959_rabbit-mthfd1-pab-30122024152144.jpg "Immunoprecipitation:MTHFD1 AntibodyNBP3-29959] -Detection of human MTHFD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFD1 antibody NBP3-29959 (lot NBP3-29959-1) used for IP at 6 µg per reaction. MTHFD1 was also immunoprecipitated by rabbit anti-MTHFD1 antibodies for blotting immunoprecipitated MTHFD1, NBP3-29959 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: MTHFD1 Antibody [NBP2-99164] - Immunofluorescence staining of MTHFD1 in 293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99164-img0001.jpg "Immunocytochemistry/Immunofluorescence: MTHFD1 Antibody [NBP2-99164] - Immunofluorescence staining of MTHFD1 in 293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.")
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP2-99164] - Immunochemical staining of human MTHFD1 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP2-99164-img0002.jpg)
![Western Blot: MTHFD1 Overexpression Lysate (Adult Normal) [NBP2-07686] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MTHFD1.](http://images.novusbio.com/fullsize/MTHFD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07686-img0001.jpg "Western Blot: MTHFD1 Overexpression Lysate (Adult Normal) [NBP2-07686] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MTHFD1.")
![Immunocytochemistry/Immunofluorescence: MTHFD1 Antibody [NBP2-97940] - Immunofluorescence staining of MTHFD1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/MTHFD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97940-img0001.jpg "Immunocytochemistry/Immunofluorescence: MTHFD1 Antibody [NBP2-97940] - Immunofluorescence staining of MTHFD1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")